Dynamic Dialysis: An Efficient Technique for Large-Volume Sample Desalting
Dynamic Dialysis: An Efficient Technique for Large-Volume Sample Desalting
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DOI：
10.1080/10826068.2014.940537
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发表时间：
2015-08-18
影响因子：
2.9
通讯作者：
Huang, Chunhong
中科院分区：
文献类型：
--
作者：
Yuan, Peng;Le, Zhen;Huang, Chunhong
透析是一种众所周知的实验室分离技术。然而，其效率通常受到透析器体积及其被动扩散方式的限制。此外，在长时间透析过程中，样品可能会沉淀和失活。为了克服这些缺点，动态透析方法进行了描述和评价。通过两个反向工作的蠕动泵进行动态透析，以分别驱动样品和缓冲液的逆流平行流动。通过记录和统计比较不同条件下截留液电导的变化来评价这种动态透析方法的效率和容量。动态方法被证明是有效的，在透析大体积的样品，其效率的变化成正比的样品流量。总之，循环样品和缓冲液可产生最高可能的浓度梯度，从而显著提高透析能力并缩短透析时间。
Dialysis is a well-known technique for laboratory separation. However, its efficiency is commonly restricted by the dialyzer volume and its passive diffusion manner. In addition, the sample is likely to be precipitated and inactive during a long dialysis process. To overcome these drawbacks, a dynamic dialysis method was described and evaluated. The dynamic dialysis was performed by two peristaltic pumps working in reverse directions, in order to drive countercurrent parallel flow of sample and buffer, respectively. The efficiency and capacity of this dynamic dialysis method was evaluated by recording and statistically comparing the variation of conductance from retentate under different conditions. The dynamic method was proven to be effective in dialyzing a large-volume sample, and its efficiency changes proportionally to the flow rate of sample. To sum up, circulating the sample and the buffer creates the highest possible concentration gradient to significantly improve dialysis capacity and shorten dialysis time.