Method based on solid-phase microextraction-high-performance liquid chromatography with UV and electrochemical detection to determine estrogenic compounds in water samples
Method based on solid-phase microextraction-high-performance liquid chromatography with UV and electrochemical detection to determine estrogenic compounds in water samples
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DOI：
10.1016/s0021-9673(02)00694-5
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发表时间：
2002-07-26
影响因子：
4.1
通讯作者：
Marcé, RM
中科院分区：
文献类型：
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作者：
Peñalver, A;Pocurull, E;Marcé, RM
采用固相微萃取(SPME)-高效液相色谱-紫外(UV)和电化学检测(ED)相结合的方法对一组雌激素类化合物进行了检测。采用改装后的高效液相色谱仪。用聚丙烯酸酯纤维(85千米)从水样中提取分析物。比较了动态和静态解吸模式，优化了固相微萃取-高效液相色谱中影响吸附和解吸过程的变量。静态解吸的回收率和峰形最好。以河水和废水为例，考察了固相微萃取-高效液相色谱-紫外可见光谱检测方法的性能。该方法能够在真实水样中以低马克数L(-1)的水平测定雌激素化合物。紫外检测法的检出限为0.3~1.1g L(-1)，ED法的检出限为0.0 6~0.0 8g L(-1)。在一家污水处理厂的样品中发现了浓度在1.9g至2.2g L(-1)之间的对雌二醇。(C)2002 Elsevier Science B.V.保留所有权利。
We determined a group of estrogenic compounds by solid-phase microextraction (SPME) coupled to high-performance liquid chromatography (HPLC) with both ultraviolet (UV) and electrochemical detection (ED). A modified liquid chromatograph was used. Polyacrylate fibers (85 km) were used to extract the analytes from the aqueous samples. Dynamic and static modes of desorption were compared and the variables affecting both absorption and desorption processes in SPME-HPLC were optimized. Static desorption gave the best recoveries and peak shapes. The performance of the SPME-HPLC-UV-ED method was checked with river water and wastewater. The method enabled estrogenic compounds to be determined at low-mug l(-1) levels in real water samples. Limits of detection were between 0.3 and 1.1 mug l(-1) using UV detection and between 0.06 and 0.08 mug l(-1) using ED. P-Estradiol was found in samples from a wastewater treatment plant at concentrations between 1.9 and 2.2 mug l(-1). (C) 2002 Elsevier Science B.V. All rights reserved.