POLLUTION OF MODERN METALWORKING FLUIDS CONTAINING BIOCIDES BY PATHOGENIC BACTERIA IN FRANCE - REEXAMINATION OF CHEMICAL TREATMENTS ACCURACY
POLLUTION OF MODERN METALWORKING FLUIDS CONTAINING BIOCIDES BY PATHOGENIC BACTERIA IN FRANCE - REEXAMINATION OF CHEMICAL TREATMENTS ACCURACY
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DOI：
10.1007/bf01719939
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发表时间：
1995-02-01
影响因子：
13.6
通讯作者：
CHAZAL, PM
中科院分区：
文献类型：
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作者：
CHAZAL, PM
在150个法国金属加工液样品中检测了致病菌污染。革兰氏阴性微生物，如沙门氏菌属，志贺氏菌属，和弧菌属。以及革兰氏阳性球菌从未分离。然而，铜绿假单胞菌和肺炎克雷伯菌等机会致病菌仍然污染这些液体，分离频率为17%的样品。这两种微生物无法在杀菌剂保护的无菌切削液中生长，甚至无法在体外存活。其他微生物的初步生长，如恶臭假单胞菌或荧光假单胞菌，这是土著微生物区系的主要部分，似乎是其生长的先决条件。这前两个假单胞菌可以抵抗三种不同类别的杀生物剂，至少在甲酰胺释放的情况下，适应其次是杀生物剂恶化。在存在三种不同类型的杀生物剂的情况下观察到抗性放大，并且在甲醛释放剂的情况下，抗性和劣化水平接近于制造商推荐的水平。这可能是为什么恶臭假单胞菌的初步生长允许在体外不同的肺炎克雷伯菌和铜绿假单胞菌的生长。由于条件致病菌的分离频率相对较高（占样本的17%），应定期对切削液进行微生物检查，以评估对人体健康的风险。戴口罩和手套仍然是推荐的，至少在法国是这样。
Pollution by pathogenic bacteria was examined in 150 French metalworking fluid samples. Gram-negative micro-organisms such as Salmonella spp., Shigella spp., and Vibrio spp. as well as Grampositive cocci were never isolated. Nevertheless opportunistic pathogens such as Pseudomonas aeruginosa and Klebsiella pneumoniae still contaminated these fluids with an isolation frequency of 17% of samples for each. These two micro-organisms failed to grow or even survive in vitro in sterile cutting fluids protected by biocides. Preliminary growth of other micro-organisms such as Pseudomonas putida or Pseudomonas fluorescens, which are the major part of the indigenous microflora, seemed to be a prerequisite for their growth. These former two Pseudomonas could resist three different classes of biocides and; at least in the case of formaldehyde-releasers, adaptation was followed by biocide deterioration. Resistance magnification was observed in the presence of the three different types of biocides and, in the case of formaldehyde releasers the resistance and deterioration levels were close to those recommended by the manufacturers. This is probably the reason why the preliminary growth of Pseudomonas putida allowed in vitro differed growth of Klebsiella pneumoniae and Pseudomonas aeruginosa. Due to relatively high isolation frequencies of opportunistic pathogens (17% of samples) periodical microbiological examination of cutting fluids should be carried out in order to evaluate risks for human health. Wearing masks and gloves is still recommended, at least in France.