Mixture Analysis of Associations between Occupational Exposure to Polycyclic Aromatic Hydrocarbons and Sperm Oxidative DNA Damage.
Mixture Analysis of Associations between Occupational Exposure to Polycyclic Aromatic Hydrocarbons and Sperm Oxidative DNA Damage.
复制标题
职业接触多环芳烃与精子氧化 DNA 损伤之间关联的混合分析。
DOI：
10.1093/annweh/wxab072
复制
发表时间：
2022
影响因子：
2.6
通讯作者：
Chang-Chien,Guo-Ping
中科院分区：
文献类型：
--
作者：
AnnaJeng,Hueiwang;Sikdar,Sinjini;Pan,Chih-Hong;Chang-Chien,Guo-Ping
目的本研究旨在确定(i)个人呼吸区多环芳烃（PAH）混合物与16种靶向PAH化合物水平与精子氧化DNA损伤之间的关系，（ii）单个PAH化合物水平与精子氧化DNA损伤之间的关系，（iii）氧化应激作为对精子遗传毒性作用的作用模式。（iv）暴露于多环芳烃混合物和/或单个多环芳烃化合物与精子DNA氧化损伤之间的任何剂量-反应关系。方法采用气相色谱-质谱联用技术对38名焦炉工人和24名对照者个人呼吸区16种靶多环芳烃进行定量分析。通过测定精子7,8-二氢-8-氧鸟嘌呤（8-oxodGuo）、精液丙二醛（MDA）和精子活性氧（ROS）水平来评估精子的氧化损伤和状态。采用贝叶斯核机回归与层次变量选择过程来确定多环芳烃混合物与精子氧化损伤生物标志物的关系。提出了一种基于多环芳烃湾区和分子量的分级变量选择方法。结果多环芳烃混合物在低百分位数（25 ~ 50）随精子8-氧过含量的增加呈阳性趋势。多环芳烃混合物的暴露与精子中丙二醛水平的增加有关。多环芳烃混合物的海湾和海湾样区域是估计多环芳烃混合物与精子氧化应激状态之间关系的最重要组。苯并[a]蒽是与MDA水平升高相关的主要单个多环芳烃化合物。结论职业接触多环芳烃混合物引起的精子DNA氧化损伤与焦炉工人丙二醛水平升高有关。最后，该研究确定了单个多环芳烃化合物苯并[a]蒽是多环芳烃混合物与精子氧化损伤之间的暗示关联的主要驱动因素。
ObjectiveThis study aimed to determine (i) associations between levels of the polycyclic aromatic hydrocarbon (PAH) mixture with 16 targeted PAH compounds in the personal breathing zone area and sperm oxidative DNA damage, (ii) associations between levels of individual PAH compounds and sperm oxidative DNA damage, (iii) oxidative stress as the mode of action for the genotoxic effects on sperm, and (iv) any dose–response relationship between exposure to the PAH mixture and/or individual PAH compounds and sperm oxidative DNA damage.MethodsSixteen targeted PAH compounds in the personal breathing zone area of 38 coke-oven workers and 24 control subjects were quantified using gas chromatography–mass spectrometry. Sperm oxidative damage and status were evaluated by measuring levels of sperm 7,8-dihydro-8-oxoguanie (8-oxodGuo), seminal malondialdehyde (MDA) and seminal reactive oxygen species (ROS). Bayesian kernel machine regression with hierarchical variable selection process was employed to determine associations of the PAH mixture and the biomarkers of sperm oxidative damage. A novel grouping approach needed for the hierarchical variable selection process was developed based on PAH bay region and molecular weight.ResultsThe PAH mixture exhibited a positive trend with increased sperm 8-oxodGuo levels at their lower percentiles (25th–50th). The exposure of the PAH mixture was associated with increased MDA levels in sperm. Bay and bay-like regions of the PAH mixture were the most important group for estimating the associations between the PAH mixture and sperm oxidative stress status. Benzo[a]anthracene was the main individual PAH compound that was associated with increased MDA levels.ConclusionSperm oxidative DNA damage induced by occupational exposure to the PAH mixture had a suggestive association with increased MDA levels in coke-oven workers. Finally, the study identified that the individual PAH compound, benzo[a]anthracene, was the primary driver for the suggestive association between the PAH mixture and sperm oxidative damage.