DETERMINATION OF ORGANIC-ACIDS IN SOIL EXTRACTS BY ION CHROMATOGRAPHY
DETERMINATION OF ORGANIC-ACIDS IN SOIL EXTRACTS BY ION CHROMATOGRAPHY
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DOI：
10.1016/0038-0717(94)00178-4
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发表时间：
1995-03-01
影响因子：
9.7
通讯作者：
LEROUX, GD
中科院分区：
文献类型：
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作者：
BAZIRAMAKENGA, R;SIMARD, RR;LEROUX, GD
低分子量有机酸在农业和森林生态系统的养分循环和植物生长中起着决定性作用。我们的研究旨在开发一种灵敏的方法来识别和量化温室条件下生长的江湖草根际土壤提取物中的有机酸。该程序包括使用阶梯梯度进行离子交换色谱分离，以及通过芳香酸的紫外吸收和脂肪酸的电导率进行定量。在单次色谱运行中，55 分钟内分离出 14 种脂肪酸，25 分钟内分离出 14 种芳香酸。该方法精密度高，脂肪酸峰面积相对标准偏差为1.50%～7.99%，芳香酸峰面积相对标准偏差为0.65%～6.37%。建议将脂肪酸方法用于单羧酸或二羧酸分析，而芳香酸方法相对于以前的程序有重大改进。我们在 quackgrass 根际中鉴定出 13 种有机酸，在含有该植物分解残留物的土壤中鉴定出 18 种有机酸。在含有庸医草残留物的土壤中发现了最大的浓度。脂肪酸浓度通常大于芳香酸浓度。甲酸和乙酸在脂肪族酸中占主导地位，而苯甲酸和对羟基苯甲酸在芳香族酸中含量最多。
Low molecular-weight organic acids play a determinant role in nutrient cycling and plant growth in agricultural and forest ecosystems. Our study was made to develop a sensitive method to identify and quantify organic acids in soil extracts from the rhizosphere of quackgrass grown under greenhouse conditions. The procedure involves ion-exchange chromatographic separation using step gradient, and quantification by UV absorption for aromatic acids and by conductivity for aliphatic acids. In a single chromatographic run, 14 aliphatic acids were separated in 55 min and 14 aromatic acids in 25 min. The methods have high precision with relative standard deviations of peak areas ranging from 1.50 to 7.99% for aliphatic acids and from 0.65 to 6.37% for aromatic acids. The method for aliphatic acids is recommended for mono- or dicarboxylic acid analysis, whereas the method for aromatic acids constitutes a major improvement over previous procedures. We identified 13 organic acids in the quackgrass rhizosphere and 18 organic acids in soil containing decomposing residues of this plant. The largest concentrations were found in soils containing quackgrass residues. Aliphatic acid concentrations were generally larger than those of aromatic acids. Formic and acetic acids dominated the aliphatic group, whereas benzoic and p-hydroxybenzoic acids were found in largest amounts among aromatic acids.