CREB activation affects mesenchymal stem cell migration and differentiation in periodontal tissues due to orthodontic force
CREB activation affects mesenchymal stem cell migration and differentiation in periodontal tissues due to orthodontic force
复制标题
由于正畸力，CREB激活影响牙周组织中间充质干细胞的迁移和分化。
DOI：
10.1016/j.biocel.2020.105862
复制
发表时间：
2020-12-01
影响因子：
4
通讯作者：
Han, Guangli
中科院分区：
文献类型：
--
作者：
Chang, Maolin;Lin, Heng;Han, Guangli
在正畸牙齿移动过程中，牙周膜细胞可分化为成骨细胞，合成牙槽骨，并影响间充质干细胞的增殖、迁移和分化，从而促进骨改建。然而，这一机制在很大程度上仍然难以捉摸。在这里，我们评估的表达CREB在张力网站的小鼠牙周膜下正畸机械应变和周期性张力应变处理的人牙周膜细胞。然后，通过功能获得和丧失分析，我们发现CREB在PDLCs中促进SDF-1和FGF 2的分泌，从而促进BMSCs的迁移和成骨分化。本研究进一步发现CREB通过与启动子区结合，转录激活FGF 2和SDF-1的表达，证实CREB是牙周膜在正畸牵张应力刺激下表达上调的基因，在正畸牵张应力诱导骨形成过程中对BMSCs的生理活性起重要调节作用。
During the orthodontic tooth movement, cells in periodontal ligament could differentiate into osteoblasts to synthesize alveolar bone as well as affect the proliferation, migration and differentiation of mesenchymal stem cells, which also contribute to bone remodeling. However, the mechanism is still largely elusive. Here, we evaluated the expression of CREB at the tension site of mouse periodontal ligament under orthodontic mechanical strain and in the cyclic tension strain treated human periodontal ligament cells. Then, through gain and loss of function analysis, we revealed that CREB in PDLCs promotes SDF-1 and FGF2 secretion, which enhance the migration and osteoblastic differentiation of BMSCs. We further discovered that CREB transcriptionally activates FGF2 and SDF-1 expressions by binding to the promoter regions.In conclusion, this study confirms that CREB is an upregulated gene in periodontal ligament under orthodontic tension strain stimulation and plays an important role in regulating BMSCs' physiological activity in orthodontic tension strain-induced bone formation.