Novel nanofibrous electrically conductive scaffolds based on poly(ethylene glycol)s-modified polythiophene and poly(ε-caprolactone) for tissue engineering applications
Novel nanofibrous electrically conductive scaffolds based on poly(ethylene glycol)s-modified polythiophene and poly(ε-caprolactone) for tissue engineering applications
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DOI：
10.1016/j.polymer.2016.11.012
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发表时间：
2016-12-19
期刊：
影响因子：
4.6
通讯作者：
Massoumi, Bakhshali
中科院分区：
文献类型：
--
作者：
Hatamzadeh, Maryam;Najafi-Moghadam, Peyman;Massoumi, Bakhshali
本研究探索了利用静电纺丝技术从AR（4）杂臂H形聚乙二醇修饰的聚噻吩[PEGs-b-（PTh）（4）]共聚物和聚己内酯（PCL）制备导电纳米纤维作为组织工程（TE）应用的支架生物材料。以PEG为原料，通过多步反应合成了两种AB（4）杂臂H型导电PEG（2000）-B-（PTh）（4）和PEG（6000）-B-（PTh）（4）。然后，通过PEG（OH）（4）与2-噻吩乙酸的Steglich酯化反应合成噻吩官能化的PEG AR（4）大分子单体（ThPEGsM）。将所得大分子单体与噻吩单体进行化学氧化共聚，得到AR（4）杂臂H型导电聚合物。将合成的改性导电聚合物和PCL的溶液静电纺丝以产生均匀的、导电的和生物相容的纳米纤维。通过场发射扫描电子显微镜（FE-SEM）和MTT法分别评估人成骨细胞MG-63的粘附、存活和增殖来证实所制造的纳米纤维的生物相容性。根据形态，导电性，亲水性，机械性能以及生物学研究，所制造的静电纺丝纳米纤维被发现作为合适的支架用于TE应用，需要电活性。(C)2016爱思唯尔有限公司版权所有。
This study explores the fabrication of electrically conductive nanofibers using electrospinning technique from AR(4) miktoarm H-shaped poly(ethylene glycol)s-modified polythiophene [PEGs-b-(PTh)(4)] copolymers and poly(e-caprolactone) (PCL) as scaffolding biomaterials for tissue engineering (TE) applications. For this purpose, two AB(4) miktoarm H-shaped conductive PEG(2000)-b-(PTh)(4) and PEG(6000)-b-(PTh)(4) were synthesized through the multistep process started from diepoxylated PEGs, and subsequently hydrolyzed to PEGs ends-caped tetraol [PEGs(OH)(4)]. Afterward, thiophene-functionalized PEGs AR(4) macromonomers (ThPEGsM) were synthesized through the Steglich esterification of PEGs(OH)(4) with 2-thiopheneacetic acid. The resultant macromonomers were subsequently used in chemical oxidation copolymerization with thiophene monomer to afford AR(4) miktoarm H-shaped conductive polymers. The solutions of the synthesized modified conductive polymers and PCL were electrospun to produce uniform, conductive, and biocompatible nanofibers. The biocompatibilities of the fabricated nanofibers were confirmed by assessing the adhesion, viability and proliferation of human osteoblast MG-63 cells using field emission scanning electron microscopy (FE-SEM) and MTT assay, respectively. According to morphology, electrical conductivity, hydrophilicity, mechanical properties as well as biological studies, the fabricated electrospun nanofibers were found as suitable scaffolds for use in TE applications that require electroactivity. (C) 2016 Elsevier Ltd. All rights reserved.