Surface hydroxyl groups regulate the osteogenic differentiation of mesenchymal stem cells on titanium and tantalum metals.
Surface hydroxyl groups regulate the osteogenic differentiation of mesenchymal stem cells on titanium and tantalum metals.
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DOI：
10.1039/c7tb00111h
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发表时间：
2017-05
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影响因子：
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通讯作者：
Mengfei Yu;Jiaxing Gong;Ying Zhou;Lingqing Dong;Yihan Lin;Liang Ma;W. Weng;Kui Cheng;Huiming W
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文献类型：
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作者：
Mengfei Yu;Jiaxing Gong;Ying Zhou;Lingqing Dong;Yihan Lin;Liang Ma;W. Weng;Kui Cheng;Huiming W
钛（Ti）和钽（Ta）金属因其良好的机械性能和良好的生物相容性而被广泛用作植入物。然而，由于复杂和多种材料表面特征（如形貌、表面化学等）的协同作用，其成骨能力的结果一直相互矛盾。关于细胞行为。在这里，我们直接比较了间充质干细胞（MSC）的成骨反应与可变表面羟基的钛和钽金属表面。虽然两种表面形貌没有发现差异，细胞粘附，增殖和成骨相关标志物的表达上调与紫外线（UV）光处理后的表面羟基（-OH）的量增加。紫外光照射前，钛对MSCs成骨分化的促进作用优于钽，而紫外光照射后，钛对MSCs成骨分化的促进作用优于钽。这些结果可能归因于不同类型的表面羟基（桥接-OH和末端-OH）的相对数量，其调节初始蛋白质吸附的构象和随后的细胞行为。我们的研究结果证明了表面羟基在介导细胞-材料相互作用中的核心作用，并暗示该界面有助于优化基于Ti和Ta的骨科和牙科植入物的骨整合。
Titanium (Ti) and tantalum (Ta) metals have been widely used as implants for their favorable mechanical features and good biocompatibility. However, the results on their osteogenic capacity have been conflicting due to the synergistic effects of complex and multiple material surface features (such as topography, surface chemistries etc.) on cellular behaviors. Here, we directly compare the osteogenic response of mesenchymal stem cells (MSCs) to Ti and Ta metal surfaces with alterable surface hydroxyl groups. Although no difference was found on both surface topographies, cellular adhesion, proliferation, and the expression of osteogenic-related markers were upregulated with the increasing amount of surface hydroxyl groups (-OH) after ultraviolet (UV) light treatment. Moreover, Ti showed better effects in promoting osteogenic differentiation of MSCs than Ta before UV light treatment, but demonstrated the opposite after UV light treatment. These results might be attributed to the comparative quantity of the distinct type of surface hydroxyl groups (bridging-OH and terminal-OH), which regulated the conformation of the initial protein adsorption and subsequent cellular behaviors. Our results demonstrate the central role of the surface hydroxyl groups in mediating cell-material interactions and implicate this interface as helping in optimizing osteointegration of Ti and Ta based orthopaedic and dental implants.