DNA-SYNTHESIS AND THE CONTROL OF EMBRYONIC GENE-EXPRESSION IN C-ELEGANS
DNA-SYNTHESIS AND THE CONTROL OF EMBRYONIC GENE-EXPRESSION IN C-ELEGANS
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DOI：
10.1016/0092-8674(88)90575-2
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发表时间：
1988-05-20
期刊：
影响因子：
64.5
通讯作者：
MCGHEE, JD
中科院分区：
文献类型：
--
作者：
EDGAR, LG;MCGHEE, JD
早期C.用显微荧光分光光度法测定了线虫胚胎。Aphidicolin显示几乎立即和完全抑制DNA合成。然后，Aphidicolin被用来研究DNA合成如何控制在肠道发育过程中不同时间出现的两种生化标志物的表达：肠道颗粒和羧酸酯酶。我们表明，标记物的表达既不是通过达到正常的DNA：细胞质比例、计数正常的DNA合成轮次数来控制的，也不是通过简单地延长细胞周期来控制的。相反，这两种肠道标志物的表达需要在肠道克隆建立后的第一个细胞周期中进行短时间的DNA合成。
DNA synthesis in each cell lineage of the early C. elegans embryo was measured using microspectrofluorimetry. Aphidicolin was shown to inhibit DNA synthesis almost instantly and completely. Aphidicolin was then used to investigate how DNA synthesis controls expression of two biochemical markers that appear at different times during gut development: gut granules and a carboxylesterase. We show that marker expression is controlled neither by reaching the normal DNA:cytoplasm ratio, by counting the normal number of rounds of DNA synthesis, nor by a simple lengthening of the cell cycle. Instead, expression of both gut markers requires a short period of DNA synthesis in the first cell cycle after the gut has been clonally established.