DTASelect and contrast: Tools for assembling and comparing protein identifications from shotgun proteomics
DTASelect and contrast: Tools for assembling and comparing protein identifications from shotgun proteomics
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DOI：
10.1021/pr015504q
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发表时间：
2002-01-01
影响因子：
4.4
通讯作者：
Yates, JR
中科院分区：
文献类型：
--
作者：
Tabb, DL;McDonald, WH;Yates, JR
复合多肽混合物的成分可以通过液相色谱法分离，串联质谱法片段化，并通过SEQUEST算法鉴定。推断混合物的来源蛋白质需要将已识别的肽重新关联。随着多肽数量的增加，这一过程变得更具挑战性。DTASelect是一个新的软件包，它可以汇编SEQUEST标识并突出显示最重要的匹配。附带的对比工具比较DTASelect从多个实验得到的结果。这两个程序提高了蛋白质组学数据分析的速度和精度。
The components of complex peptide mixtures can be separated by liquid chromatography, fragmented by tandem mass spectrometry, and identified by the SEQUEST algorithm. Inferring a mixture's source proteins requires that the identified peptides be reassociated. This process becomes more challenging as the number of peptides increases. DTASelect, a new software package, assembles SEQUEST identifications and highlights the most significant matches. The accompanying Contrast tool compares DTASelect results from multiple experiments. The two programs improve the speed and precision of proteomic data analysis.