CHOLECYSTOKININ-RELEASING, GALANIN-RELEASING, AND CORTICOTROPIN-RELEASING FACTOR-LIKE IMMUNOREACTIVE PROJECTIONS FROM THE NUCLEUS OF THE SOLITARY TRACT TO THE PARABRACHIAL NUCLEUS IN THE RAT
CHOLECYSTOKININ-RELEASING, GALANIN-RELEASING, AND CORTICOTROPIN-RELEASING FACTOR-LIKE IMMUNOREACTIVE PROJECTIONS FROM THE NUCLEUS OF THE SOLITARY TRACT TO THE PARABRACHIAL NUCLEUS IN THE RAT
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DOI：
10.1002/cne.902930405
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发表时间：
1990-03-22
影响因子：
2.5
通讯作者：
SAPER, CB
中科院分区：
文献类型：
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作者：
HERBERT, H;SAPER, CB
臂旁核（PB）是上行内脏传入信息从孤立束核（NTS）到前脑的主要中继。我们通过荧光示踪剂的联合逆行转运和免疫组织化学对甘丙氨酸（GAL）、胆囊收缩素（CCK）和促肾上腺皮质激素释放因子（CRF）进行检测，研究了孤立-臂旁神经事件的化学组织。每个肽显示出独特的免疫反应染色模式。gal样免疫反应性（-ir）纤维在“腰部”区、外外侧PB内侧、中外侧PB亚核和背外侧PB亚核中最为突出。在内侧和外内侧PB亚核中可见额外的GAL-ir神经支配。髓核周围主要在背外侧、上外侧和极外侧观察到。CCK-ir纤维和终端在外外侧PB的外侧部分最为突出；中央外侧PB也有一些较弱的标记。CCK-ir细胞体几乎完全局限于上外侧PB和“腰部”区域，尽管在Kolliker-Fuse核中可见少量细胞。CRF-ir末端纤维的分布与GAL相似，但在PB背侧和中央外侧外侧以及外内侧和Kolliker-Fuse亚核的末端标记少得多。CRF-ir细胞数量最多的部位是外侧壁背侧和外侧壁外侧；在眼侧，散在的CRF-ir神经元主要分布在中央外侧脑区。在PB中注射荧光示踪剂Fast Blue，绘制双标记神经元在NTS中的分布。GAL-ir细胞主要位于NTS内侧亚核；34%的GAL-ir细胞为同侧双标记，7%为对侧双标记。相反，17%的逆行标记的细胞同侧和16%的对侧是GAL-ir。CCK-ir神经元以NTS背内侧亚核和后脑区外缘最多。在CCK-ir细胞中，68%的同侧和10%的对侧NTS细胞是双标记的，而15%和10%的逆行标记细胞是CCK-ir。在死后区域，36%的CCK-ir细胞和9%的Fast Blue细胞被双重标记。CRF-ir神经元更广泛地分布于NTS内侧、背内侧和腹外侧亚核，但双标记细胞主要分布于NTS内侧。在NTS的CRF-ir细胞中，26%的同侧和8%的对侧被PB注射逆行标记。相反，在NTS逆行标记的细胞中，4%的同侧和6%的对侧是CRF-ir。我们的结果表明，NTS事件的功能特异性可能是通过它们在特定的PB亚核中的选择性终止来维持的。此外，在这些通路中发现的神经肽可能为特定类型的内脏感觉信息传递到PB提供化学编码。
The parabrachial nucleus (PB) is the main relay for ascending visceral afferent information from the nucleus of the solitary tract (NTS) to the forebrain. We examined the chemical organization of solitary-parabrachial afferents by using combined retrograde transport of fluorescent tracers and immunohistochemistry for galanin (GAL), cholecystokinin (CCK), and corticotropin-releasing factor (CRF). Each peptide demonstrated a unique pattern of immunoreactive staining. GAL-like immunoreactive (-ir) fibers were most prominent in the "waist" area, the inner portion of external lateral PB, and the central and dorsal lateral PB subnuclei. Additional GAL-ir innervation was seen in the medial and external medial PB subnuclei. GAL-ir perikarya were observed mainly rostrally in the dorsal lateral, superior lateral, and extreme lateral PB. CCK-ir fibers and terminals were most prominent in the outer portion of the external lateral PB; some weaker labeling was also present in the central lateral PB. CCK-ir cell bodies were almost exclusively confined to the superior lateral PB and the "waist" area, although a few cells were seen in the Kolliker-Fuse nucleus. The distribution of CRF-ir terminal fibers in general resembled that of GAL, but showed considerably less terminal labeling in the lateral parts of the dorsal and central lateral PB, and the external medial and Kolliker-Fuse subnuclei. The CRF-ir cells were most numerous in the dorsal lateral PB and the outer portion of the external lateral PB; rostrally, scattered CRF-ir neurons were seen mainly in the central lateral PB. After injecting the fluorescent tracer Fast Blue into the PB, the distribution of double-labeled neurons in the NTS was mapped. GAL-ir cells were mainly located in the medial NTS subnucleus; 34% of GAL-ir cells were double-labeled ipsilaterally and 7% contralaterally. Conversely, 17% of the retrogradely labeled cells ipsilaterally and 16% contralaterally were GAL-ir. CCK-ir neurons were most numerous in the dorsomedial subnucleus of the NTS and the outer rim of the area postrema. Of the CCK-ir cells, 68% in the ipsilateral and 10% in the contralateral NTS was double-labeled, whereas 15% and 10%, respectively, of retrogradely labeled cells were CCK-ir. In the area postrema, 36% of the CCK-ir cells and 9% of the Fast Blue cells were double-labeled. CRF-ir neurons were more widely distributed in the medial, dorsomedial, and ventrolateral NTS subnuclei, but double-labeled cells were mainly seen in the medial NTS. Of CRF-ir cells in the NTS, 26% ipsilaterally and 8% contralaterally were retrogradely labeled by the PB injections. Conversely, of retrogradely labeled cells in the NTS, 4% ipsilaterally and 6% contralaterally were CRF-ir. Our results suggest that the functional specificity of NTS afferents may be maintained by their selective termination in particular PB subnuclei. In addition, the neuropeptides found in these pathways may provide chemical coding for the relay of specific types of visceral sensory information to the PB.