A multiphase model for tissue construct growth in a perfusion bioreactor
A multiphase model for tissue construct growth in a perfusion bioreactor
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DOI：
10.1093/imammb/dqp003
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发表时间：
2010-06-01
影响因子：
1.1
通讯作者：
Byrne, H. M.
中科院分区：
文献类型：
--
作者：
O'Dea, R. D.;Waters, S. L.;Byrne, H. M.
使用由Lemon等人提出的三相连续模型（2006，Multiphase modeling of tissue growth using the theory of mixtures.数学生物学杂志，52，571-594）以代表细胞群（和伴随的细胞外基质）、培养基和多孔支架。将生物反应器系统建模为含有细胞接种的刚性多孔支架（组织构建体）的2D通道，该支架灌注有培养基。该研究集中在（i）细胞-细胞和细胞-支架相互作用和（ii）机械转导机制对构建体组成的影响。的模型方程的数值和分析分析，并根据细胞聚集和排斥的相对重要性，显着不同的细胞运动被揭示。此外，由于细胞密度、压力和剪切应力介导的组织生长的机械转导效应显示出在所得构建体的组成中产生质的差异。我们的模拟结果表明，该模型配方（结合适当的实验数据）有可能提供一种手段，确定在细胞群中的主导调节刺激。
The growth of a cell population within a rigid porous scaffold in a perfusion bioreactor is studied, using a three-phase continuum model of the type presented by Lemon et al. (2006, Multiphase modelling of tissue growth using the theory of mixtures. J. Math. Biol., 52, 571-594) to represent the cell population (and attendant extracellular matrix), culture medium and porous scaffold. The bioreactor system is modelled as a 2D channel containing the cell-seeded rigid porous scaffold (tissue construct) which is perfused with culture medium. The study concentrates on (i) the cell-cell and cell-scaffold interactions and (ii) the impact of mechanotransduction mechanisms on construct composition. A numerical and analytical analysis of the model equations is presented and, depending upon the relative importance of cell aggregation and repulsion, markedly different cell movement is revealed. Additionally, mechanotransduction effects due to cell density, pressure and shear stress-mediated tissue growth are shown to generate qualitative differences in the composition of the resulting construct. The results of our simulations indicate that this model formulation (in conjunction with appropriate experimental data) has the potential to provide a means of identifying the dominant regulatory stimuli in a cell population.