In vivo imaging of evoked calcium responses indicates the intrinsic axonal regenerative capacity of zebrafish
In vivo imaging of evoked calcium responses indicates the intrinsic axonal regenerative capacity of zebrafish
复制标题
诱发钙反应的体内成像表明斑马鱼固有的轴突再生能力
DOI：
10.1096/fj.201802649r
复制
发表时间：
2019-06-01
期刊：
影响因子：
4.8
通讯作者：
Hu, Bing
中科院分区：
文献类型：
--
作者：
Chen, Min;Huang, Rong-Chen;Hu, Bing
钙离子是神经系统中的重要信使，但其在轴突再生中的具体作用尚未得到充分研究。为了澄清这一点，我们构建了一个非侵入性的体内钙成像模型的斑马鱼Mauthner细胞和监测轴突再生过程中的亚细胞钙动力学。使用钙指示剂GCamp 6 f，我们观察到再生长度与轴突切断前诱发钙反应的峰值幅度相关，这表明诱发钙反应可能作为诱发神经元活性和轴突再生能力的有用指标。为了研究这种可能性，我们过表达内向整流钾通道蛋白Kir2.1a，以降低Mauthner神经元的活性，并发现钙反应的抑制与轴突再生减少相关。与此相反，戊四氮和敲除钠电压门控通道α亚基1基因的治疗增加了钙反应，从而增强了轴突再生。因此，我们的研究结果增加了对神经活动与脊椎动物轴突再生之间相关性的理解。陈美，黄河，巴西-地C.的方法，扬湖，澳-地问：Ren，D.- L.，Hu，B.诱发钙反应的体内成像表明斑马鱼的内在轴突再生能力。
Calcium is an important messenger in the neuronal system, but its specific role in axonal regeneration has not been fully investigated. To clarify it, we constructed a noninvasive in vivo calcium-imaging model of zebrafish Mauthner cells and monitored subcellular calcium dynamics during axonal regeneration. Using the calcium indicator GCamp6f, we observed that the regenerative length correlated with the peak amplitude of the evoked calcium response before axotomy, which suggested that the evoked calcium response might serve as a useful indicator of evoked neuronal activity and axonal regenerative capacity. To investigate this possibility, we overexpressed an inward rectifying potassium channel protein, Kir2.1a, to decrease the Mauthner neuronal activity and found that the inhibition of the calcium response correlated with decreased axonal regeneration. In contrast, treatment of pentylenetetrazol and knockout of the sodium voltage-gated channel alpha subunit 1 gene increased the calcium response and thus enhanced axonal regeneration. Our results therefore increased the understanding of the correlation between the neural activity and the vertebrate axonal regeneration.-Chen, M., Huang, R.-C., Yang, L.-Q., Ren, D.-L., Hu, B. In vivo imaging of evoked calcium responses indicates the intrinsic axonal regenerative capacity of zebrafish.