Human oropharyngeal lesions with a defective Epstein-Barr virus that disrupts viral latency.
Human oropharyngeal lesions with a defective Epstein-Barr virus that disrupts viral latency.
复制标题
人类口咽部病变带有缺陷的 Epstein-Barr 病毒，可破坏病毒潜伏期。
DOI：
10.1093/infdis/168.6.1349
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Sixbey,JW
中科院分区：
文献类型：
--
作者：
Gan,YJ;Shirley,P;Zeng,Y;Sixbey,JW
EB病毒（EBV）是一种感染后主要潜伏的人类疱疹病毒，可通过实验室菌株P3 HR-1培养物中缺失、重排的EBV DNA诱导复制。由于粘膜表面允许EB病毒复制，我们用BamH Ⅰ W和Z EBVDNA限制性片段的异常并列作为标记，检测了70名中国和5名美国患者的101份口腔活检组织中是否存在组织培养缺陷病毒（WZhet）的天然对应物。聚合酶链反应分析显示，在49例含有EBV DNA的口腔活检组织中，12例（24%）有重排的WZ片段：7例EBV阳性上皮发育不良或癌中的3例（42%），16例毛状白斑中的6例（38%），25例非恶性唾液腺活检组织中的3例（12%）。通过原位细胞杂交和选择WZhe阳性病变中基因组线性构型的证明证实了伴随的病毒复制。这些发现表明，具有破坏EBV潜伏期的不寻常特性的缺陷型EBV在自然感染中普遍存在，并可能导致EBV的致病性多样性。
The Epstein-Barr virus (EBV), a human herpesvirus that is predominantly latent after infection, can be induced to replicate by deleted, rearranged EBV DNA from cultures of laboratory strain P3HR-1. Because mucosal surfaces are permissive ofEBV replication, 101 oral biopsies from 70 Chinese and 5 American patients were examined for natural counterparts to tissue culture defective virus (WZhet), using as marker the abnormal juxtaposition ofBamHI Wand Z EBV DNA restriction fragments. Of the 49 oral biopsies that contained EBV DNA, 12 (24%) had the rearranged WZ fragment by polymerase chain reaction analysis: 3 (42%) of7 EBV-positive epithelial dysplasias or carcinomas, 6 (38%) of 16 hairy leukoplakias, and 3 (12%) of 25 nonmalignant salivary gland biopsies. Accompanying viral replication was confirmed by in situ cytohybridization and demonstration of the linear configuration of the genome in select WZhetpositive lesions. These findings indicate that defectiveEBV with the unusual property of disrupting EBV latency is prevalent in natural infections and may contribute to EBV's pathogenic diversity.