PACEMAKER ACTIVITY AND MITOSIS IN CULTURES OF NEWBORN RAT HEART VENTRICLE CELLS
PACEMAKER ACTIVITY AND MITOSIS IN CULTURES OF NEWBORN RAT HEART VENTRICLE CELLS
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DOI：
10.1016/0014-4827(66)90427-7
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发表时间：
1966-01-01
影响因子：
3.7
通讯作者：
STRASSER, FF
中科院分区：
文献类型：
--
作者：
MARK, GE;STRASSER, FF
描述了一种可重复的培养系统，其中单个新生大鼠心室细胞发育成分化的、同步跳动的网络，并继续发挥作用数周，没有去分化的证据，也没有跳动率的损失。与只有心脏的“起搏器”细胞可以启动跳动的公认观点相反，结果表明，新生大鼠心室中的所有肌肉细胞似乎都能够独立且自发地进行组织培养收缩。不同细胞的跳动速率差异很大。讨论了这对于心脏胚胎发育的重要性。连接的肌肉细胞群同步收缩，跳动最快的细胞充当起搏器。跳动同步所需的原生质连接不需要直接从一个肌肉细胞到另一个肌肉细胞，而是可以通过非肌肉细胞（可能是内皮细胞来源）进行。细胞接触时的同步（如高分辨率相差显微镜所定义）是快速的，但不是瞬时的。心肌细胞在继续跳动的同时可能会发生有丝分裂。有丝分裂似乎被修改以确保细胞在此过程中作为组织单位的一部分的持续功能。观察到所有心肌细胞的细胞核周围都有双层膜，用相差显微镜清晰可见。
A reproducible culture system was described in which single newborn rat heart ventricle cells develop into a differentiated, synchronously-beating network and continue to function for many weeks with no evidence of dedifferentiation and no loss of beating rate. Contrary to the accepted idea that only the "pacemaker" cells of the heart may initiate beating, results indicate that all muscle cells in the newborn rat heart ventricle appear to be capable of independent and spontaneous contraction of tissue culture. The rate of beating varies widely from cell to cell. The significance of this in relation to embryological development of the heart was discussed. Groups of connected muscle cells contract in synchrony, the fastest beating cell acting as pacemaker. The protoplasmic connection necessary for synchronization of beating need not be directly from one muscle cell to another, but may be made through the non-muscle cells, probably of endothelial origin. Synchronization upon cell contact (as defined by high resolution phase microscopy) is rapid but not instantaneous. Heart muscle cells may undergo mitosis while continuing to beat. The mitosis appears to be modified to assure the continued function of the cell as part of a tissue unit during this process. A double membrane surrounding the nucleus of all heart muscle cells, and clearly visible with the phase microscope, was observed.