Serum protein marker panel for predicting preeclampsia
Serum protein marker panel for predicting preeclampsia
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用于预测先兆子痫的血清蛋白标记物组
DOI：
10.1016/j.preghy.2018.01.009
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发表时间：
2018-10-01
影响因子：
2.2
通讯作者：
Li, Yulin
中科院分区：
文献类型：
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作者：
Cui, Lifeng;Shu, Chang;Li, Yulin
背景：先兆子痫是一种妊娠期多系统疾病，目前尚无有效的治疗方法。先兆子痫的诊断基于临床表现和常规实验室检查。目的：本研究旨在确定诊断先兆子痫的血清蛋白标志物并预测其严重特征。研究设计：本研究总共纳入了 172 名孕妇，其中 110 名先兆子痫受试者和 62 名血压正常受试者。使用Luminex多重免疫分析和ELISA评估11种血清蛋白（VEGF、sFlt-1、sEndoglin、PlGF、sEGFR、催乳素、PTX3、PAI-1、NGAL、IL-27、COX-2）。结果：7种蛋白（sFlt-1、VEGF、sEndoglin、sEGFR、PlGF、NGAL、COX-2）的水平 与先兆子痫相关，并且 4 个蛋白（VEGF、sEndoglin、PlGF、sEGFR）被确定为与先兆子痫相关的独立因素。三种蛋白（sEndoglin、PTX3、sFlt-1）的水平与先兆子痫的严重特征相关，并且三个变量（血清肌酐、血小板计数和sEndoglin）被确定为预测先兆子痫的严重特征的独立因素。 结论：血清蛋白标志物（VEGF、sEndoglin、PlGF、sEGFR）和临床变量的组合 （血清肌酐、血小板计数和内皮糖蛋白）可分别用作诊断先兆子痫及其严重特征的分析工具。血清sEGFR是子痫前期的一种新型生物标志物，可能参与子痫前期的发病机制。
Background: Preeclampsia is a multi-system disorder in pregnancy which has no effective treatment. The diagnosis of preeclampsia is based on clinical presentation and routine laboratory tests. Objective: This study aimed at identifying serum protein markers for diagnosis of preeclampsia and predicting its severe features.Study design: In total, 172 pregnant women were enrolled in this study including 110 subjects with preeclampsia and 62 normotensive subjects. Eleven serum proteins (VEGF, sFlt-1, sEndoglin, PlGF, sEGFR, prolactin, PTX3, PAI-1, NGAL, IL-27, COX-2) were assessed using Luminex multiplex immunoassay and ELISA.Results: The levels of seven proteins (sFlt-1, VEGF, sEndoglin, sEGFR, PlGF, NGAL, COX-2) correlated with preeclampsia, and 4 proteins (VEGF, sEndoglin, PlGF, sEGFR) were identified as independent factors associated with preeclampsia. The levels of three proteins (sEndoglin, PTX3, sFlt-1) correlated with severe features of preeclampsia, and three variables (serum creatinine, platelet count and sEndoglin) were identified as in- dependent factors in predicting severe features of preeclampsia.Conclusions: A combination of serum protein markers (VEGF, sEndoglin, PlGF, sEGFR) and clinical variables (serum creatinine, platelet count and sEndoglin) could be used as analytical tool in diagnosis of preeclampsia and its severe features, respectively. Serum sEGFR, a novel biomarker in preeclampsia, may be involved in the pathogenesis of preeclampsia.