Electrophysiological properties of human glia
Electrophysiological properties of human glia
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人类神经胶质细胞的电生理特性
DOI：
10.1016/0166-2236(82)90034-0
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发表时间：
1982
影响因子：
15.9
通讯作者：
Selwyn Picker
中科院分区：
文献类型：
--
作者：
Selwyn Picker
这种制备可行脑切片的方法，表现出与体内研究中发现的一些脑切片类似的电生理特性，已经允许对人类神经胶质细胞进行智能细胞记录。通过记录电极离子迁移标记物（如辣根过氧化物酶）以获得所研究细胞的阳性形态学鉴定，使该方法更加有效。越来越多的结果表明，这种方法有潜力获得关于正常神经胶质功能和人类大脑癫痫和神经胶质肿瘤病理生理学的新信息。在哺乳动物的大脑中，gila的数量是神经元的10倍。一般来说，大脑越大，gila与神经元的比例就越大，这表明gila应该具有重要的功能。然而，神经生物学家一直没有发现它们的生理意义，对它们功能的猜测范围从认为它们仅仅是一种“神经胶质瘤”（神经胶质），到在学习和记忆中发挥重要作用。库菲耶和他的同事们进行的细致的研究表明，gila在无脊椎动物Icech的中枢神经系统中对K'具有独家渗透性，这为gila提供了第一个定量的生理数据。这一发现，以及最近引进的技术（如示踪方法、免疫学、酶学、冷冻断裂）对神经胶质细胞研究的适用性，使人们对这些细胞产生了浓厚的兴趣。
The method for preparing viable brain slices that exhibit electrophysiologica [properties similar to some of those found in in-vivostudies has permitted intmcelluhtr recording from human glia. The method is made more powerful by iontophoresing a marker (eg horseradish peroxidase) through the recording electrode to obtain positive morphological identification of the cell being studied. Accumulating results demonstrate the potential of this method for gaining new information about normal glial function and the pathophysiology of epilepsy and glial tumors in the haman brain.In the mammalian brain, gila outnumber neurons ten to one, In general the larger the brain the greater the ratio of gila to neurons, suggesting that gila should have an important function. Yet their physiological significance has eluded neurobiologists and speculation about their function has ranged from considering them to be merely a'nerve cement'(neuroglia), to having an important role in learning and memoryL The elegant studies by Kuffier and coworkers 5, demonstrating that gila have an exclusive permeability to K'in the CNS of the invertebrate Icech, provided the first quantitative physiological data about gila. This discovery, and the applicability of recently introduced techniques (eg tracer methods, immunological, enzymological, freeze fracture) to the study of glia are responsible for the burgeoning interest in these cells.
人和豚鼠的神经胶质膜电位及其与 [K ]o 的关系。
影响因子：
4.1
作者：
Picker,S;Pieper,CF;Goldring,S
通讯作者：
Goldring,S