DETECTION OF TOBACCO RATTLE VIRUS BY REVERSE TRANSCRIPTION AND POLYMERASE CHAIN-REACTION
DETECTION OF TOBACCO RATTLE VIRUS BY REVERSE TRANSCRIPTION AND POLYMERASE CHAIN-REACTION
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DOI：
10.1016/0166-0934(92)90007-z
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发表时间：
1992-10-01
影响因子：
3.1
通讯作者：
ROBINSON, DJ
中科院分区：
文献类型：
--
作者：
ROBINSON, DJ
一种反转录和聚合酶链式反应相结合的检测方法在受感染植物的提取物中特异性地检测到烟草骚乱病毒RNA。它检测到了广泛的血清学变异和典型的非颗粒产生NM型分离株。该方法的灵敏度足以从10 ng总核酸中检测到TRV RNA，或从60杯感染叶片组织中检测到相对粗略的核酸制剂。
An assay involving reverse transcription followed by polymerase chain reaction specifically detected tobacco rattle virus RNA in extracts of infected plants. It detected a wide range of serological variants and typical non-particle producing NM-type isolates. The sensitivity of the method was sufficient to detect TRV RNA in 10 ng total nucleic acid from an infected plant, or in a relatively crude nucleic acid preparation from 60 mug infected leaf tissue.