Prevalence and Phylogenetic Analysis of Human Bocaviruses 1-4 in Pediatric Patients with Various Infectious Diseases.
Prevalence and Phylogenetic Analysis of Human Bocaviruses 1-4 in Pediatric Patients with Various Infectious Diseases.
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人类博卡病毒1-4型在患有各种传染病的儿科患者中的流行率和系统发育分析
DOI：
10.1371/journal.pone.0160603
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Zhao L
中科院分区：
文献类型：
--
作者：
Zhao M;Zhu R;Qian Y;Deng J;Wang F;Sun Y;Dong H;Liu L;Jia L;Zhao L
由人类博卡病毒1-4（HBoV 1 -4）引起的病毒感染比以前认为的更复杂。进行了一项回顾性的大规模研究，以探讨患有各种感染性疾病的儿科患者中HBoV 1 -4的患病率并描绘其系统发育特征。对从患有各种感染性疾病的儿科患者收集的来自四种标本类型的临床样品，包括4，941份呼吸道、2，239份脑脊液（CSF）、2，619份血清和1，121份粪便标本，进行了HBoV 1 -4筛查。然后扩增每个样本中的690-nt片段并测序用于系统发育分析。评价了具有不同标本类型的HBoV阳性患者的临床特征。约1.2%的患者被证实为HBoV阳性，其中胃肠道感染患者的阳性率最高（2.2%），其次是呼吸道（1.65%），中枢神经系统（0.8%）和血液系统感染（0.2%）。在8年的时间里，HBoV 1的单一遗传谱系在儿童中传播，而通过HBoV 2A和HBoV 2B之间的基因型内重组的新的HBoV 2簇是普遍的。一些患者的呼吸道和血清标本或粪便标本为HBoV 1阳性。几例病例在出现呼吸道感染后变为HBoV 1阳性，而几例病例仅在CSF和血清标本中而不是呼吸道标本中对HBoV 2呈阳性。HBoVl的单一遗传谱系被推测为呼吸道感染的病毒病原体，并引起共病感染和急性胃肠炎。此外，一个新的HBoV 2簇在中国流行，它可能通过呼吸道以外的部位感染宿主。
Viral infections caused by human bocaviruses 1–4 (HBoV1-4) are more complicated than previously believed. A retrospective, large-scale study was undertaken to explore the prevalence of HBoV1-4 in pediatric patients with various infectious diseases and delineate their phylogenetic characteristics. Clinical samples from four specimen types, including 4,941 respiratory, 2,239 cerebrospinal fluid (CSF), 2,619 serum, and 1,121 fecal specimens, collected from pediatric patients with various infectious diseases were screened for HBoV1-4. A 690-nt fragment in each specimen was then amplified and sequenced for phylogenetic analysis. Clinical characteristics of HBoV-positive patients with different specimen types available were evaluated. Approximately 1.2% of patients were confirmed as HBoV-positive, with the highest positive rate in patients with gastrointestinal infection (2.2%), followed by respiratory (1.65%), central nervous system (0.8%), and hematological infections (0.2%). A single genetic lineage of HBoV1 circulated among children over the 8-year period, while a new cluster of HBoV2, via intra-genotype recombination between HBoV2A and HBoV2B, was prevalent. Some patients had HBoV1-positive respiratory and serum specimens or fecal specimens. Several cases became HBoV1-positive following the appearance of respiratory infection, while several cases were positive for HBoV2 only in CSF and serum specimens, rather than respiratory specimens. A single genetic lineage of HBoV1 is speculated as a viral pathogen of respiratory infection and causes both comorbid infection and acute gastroenteritis. Additionally, a new cluster of HBoV2 is prevalent in China, which may infect the host through sites other than the respiratory tract.