Neuroprotective effects of Yi-Gan San against beta amyloid-induced cytotoxicity on rat cortical neurons
Neuroprotective effects of Yi-Gan San against beta amyloid-induced cytotoxicity on rat cortical neurons
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DOI：
10.1016/j.pnpbp.2008.07.006
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发表时间：
2008-10
影响因子：
5.6
通讯作者：
M. Tateno;W. Ukai;Takafumi Ono;S. Saito;E. Hashimoto;Toshikazu Saito
中科院分区：
文献类型：
--
作者：
M. Tateno;W. Ukai;Takafumi Ono;S. Saito;E. Hashimoto;Toshikazu Saito
简介 最近的临床研究表明，一甘散（YGS，日语为 Yokukan-San）是一种中草药，可以缓解各种与痴呆症相关的症状。然而，中草药很少得到科学研究，YGS 的潜在机制仍不清楚。在本研究中，我们在大鼠皮质神经元原代培养物中研究了 YGS 对 β 淀粉样蛋白 (Aβ) 诱导的细胞毒性的影响。方法将大鼠胚胎制备的皮质神经元在存在或不存在 YGS 的情况下暴露于 Aβ。通过使用 MTT 测定和 LDH 测定，以对照（未暴露的神经元）的百分比来测量 YGS 的保护作用。结果，在 20 μM 及更高剂量下，YGS 显着减少了存活皮质神经元的数量。在 20 μM Aβ 存在的情况下，YGS 浓度为 10−5g/L (W/V) 及更高浓度可显着增加存活神经元的数量。结论我们的研究证明了益肝散对 Aβ 诱导的细胞毒性具有神经保护作用。由于根据传统草药信念，YGS 很可能不是通过单一成分而是通过多种草药成分的混合物发挥其临床作用，因此这种神经保护作用的真正机制仍不清楚。然而，我们的结果表明，这种中草药可能是治疗与痴呆相关的临床症状的一种有价值的治疗方法，具有较少的副作用，并且可能对老年人有额外的神经保护作用。
INTRODUCTIONRecent clinical studies have demonstrated that Yi-Gan San (YGS, Yokukan-San in Japanese), a Chinese herbal medicine, alleviates various dementia-related symptoms. However, Chinese herbal medicines have rarely been investigated scientifically and the underlying mechanism of YGS remains uncertain. In this study, we investigated the effect of YGS on beta amyloid protein (Aβ)-induced cytotoxicity in a primary culture of rat cortical neurons.METHODSCortical neurons prepared from rat embryos were exposed to Aβ in the presence or absence of YGS. The protective effect of YGS was measured as the % of control (unexposed neurons) by using MTT assay and LDH assay.RESULTSAβ significantly decreased the number of surviving cortical neurons at a dose of 20 μM and higher. In the presence of 20 μM Aβ, YGS concentrations of 10−5g/L (W/V) and higher significantly increased the number of viable neurons.CONCLUSIONOur study demonstrated a neuroprotective effect of Yi-Gan San against Aβ-induced cytotoxicity. Since according to traditional herbal medicine beliefs, YGS most likely exerts its clinical effects not through a single constituent but as a mixture of several herbal ingredients, the true mechanism of this neuroprotective action remains unclear. However, our results suggest that this Chinese herbal medicine might be a valuable treatment for clinical symptoms associated with dementia having fewer side effects and possible additional neuroprotective effects in the elderly.