Diffusive tail anchorage determines velocity and force produced by kinesin-14 between crosslinked microtubules.
Diffusive tail anchorage determines velocity and force produced by kinesin-14 between crosslinked microtubules.
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DOI：
10.1038/s41467-018-04656-0
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发表时间：
2018-06-07
影响因子：
16.6
通讯作者：
Diez S
中科院分区：
文献类型：
--
作者：
Lüdecke A;Seidel AM;Braun M;Lansky Z;Diez S
有丝分裂纺锤体的形态和功能依赖于马达蛋白，马达蛋白使微管相互交联并相互移动。其中包括Kinesin-14s，如NCD，它通过其非进行性运动域与一个微管相互作用，并通过其扩散的尾域与另一个微管相互作用，后者允许蛋白质沿微管表面滑动。关于尾部结构域对蛋白质性能的影响，人们知之甚少。在这里，我们发现NCD尾域的扩散锚定对速度和力有很大的影响。尾域滑移使速度从2 70 nm S−1降至6 0 nm S−1，并将作用力从几个皮牛顿范围降低到亚皮牛顿范围。这些发现挑战了这样一种观点，即在有丝分裂过程中，Kinesin-14可能会作为其他交联型马达的拮抗剂，例如Kinesin-5。相反，它暗示了Kinesin-14作为一种柔性元件的作用，它柔韧地滑动和交联微管，以促进有丝分裂纺锤体的重塑。Kinesin-14s，如NCD，通过其非进行性运动结构域和扩散的尾部结构域与微管相互作用，但尾部结构域对运动性能的影响尚不清楚。在这里，作者表明，尾域滑移限制了NCD产生的速度和力，这表明它起到了光滑交联剂的作用。
Form and function of the mitotic spindle depend on motor proteins that crosslink microtubules and move them relative to each other. Among these are kinesin-14s, such as Ncd, which interact with one microtubule via their non-processive motor domains and with another via their diffusive tail domains, the latter allowing the protein to slip along the microtubule surface. Little is known about the influence of the tail domains on the protein’s performance. Here, we show that diffusive anchorage of Ncd’s tail domains impacts velocity and force considerably. Tail domain slippage reduced velocities from 270 nm s−1 to 60 nm s−1 and forces from several piconewtons to the sub-piconewton range. These findings challenge the notion that kinesin-14 may act as an antagonizer of other crosslinking motors, such as kinesin-5, during mitosis. It rather suggests a role of kinesin-14 as a flexible element, pliantly sliding and crosslinking microtubules to facilitate remodeling of the mitotic spindle. Kinesin-14s, such as Ncd, interact with microtubules with their non-processive motor domains and their diffusive tail domains, but the influence of the tail domains on motor performance is not known. Here the authors show that tail domain slippage limits the velocities and forces generated by Ncd, suggesting it acts as a slippery crosslinker.
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影响因子：
2.7
作者：
Oliveira, Catarina R.;Lemaitre, Regis;Tanaka, Elly M.
通讯作者：
Tanaka, Elly M.
DOI：
10.1083/jcb.200910125
发表时间：
2010-05-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hentrich C;Surrey T
通讯作者：
Surrey T
影响因子：
64.5
作者：
Molodtsov, Maxim I.;Mieck, Christine;Vaziri, Alipasha
通讯作者：
Vaziri, Alipasha
影响因子：
3.3
作者：
Cai, Shang;Weaver, Lesley N.;Walczak, Claire E.
通讯作者：
Walczak, Claire E.
影响因子：
64.5
作者：
Lansky, Zdenek;Braun, Marcus;Diez, Stefan
通讯作者：
Diez, Stefan