Physical characterization of Caulobacter crescentus flagella
Physical characterization of Caulobacter crescentus flagella
复制标题
新月柄杆菌鞭毛的物理特性
作者：
C. Lagenaur;N. Agabian
从新月茎杆菌CB13B1a分离的完整鞭毛中分离出两种蛋白，表观分子量分别为28000和25000。在Ouchterlony双免疫扩散试验中，两种蛋白相互完全交叉反应，并与纯化鞭毛完全交叉反应。分离的蛋白质的氨基酸组成彼此相似，但排除了任何前体-产物关系。从许多非运动突变体中缺失25000和28000分子量的蛋白质，以及这些蛋白质在运动逆转物中同时重新出现，进一步证明了这两种蛋白质与鞭毛结构的关系。
Preparations of intact flagella isolated from Caulobacter crescentus CB13B1a were found to contain two protein species of apparent molecular weights 28,000 and 25,000. Both proteins cross-reacted completely with each other and with purified flagella in Ouchterlony double-immunodiffusion assays. The amino acid compositions of the isolated proteins were similar to one another but precluded any precursor-product relationship. Absence of both the 25,000- and 28,000-molecular-weight proteins from a number of nonmotile mutants and the simultaneous reappearance of these proteins in a motile revertant provide further evidence of the relationship of these two proteins to flagellar structure.