1F7, a novel cell surface molecule, involved in helper function of CD4 cells.
1F7, a novel cell surface molecule, involved in helper function of CD4 cells.
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DOI：
10.4049/jimmunol.143.11.3430
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发表时间：
1989-12
影响因子：
4.4
通讯作者：
C. Morimoto;Y. Torimoto;G. Levinson;C. Rudd;M. Schrieber;N. Dang;N. Letvin;S. Schlossman
中科院分区：
文献类型：
--
作者：
C. Morimoto;Y. Torimoto;G. Levinson;C. Rudd;M. Schrieber;N. Dang;N. Letvin;S. Schlossman
我们已经开发了一种单克隆抗体，抗1F 7，抑制可溶性Ag驱动的T细胞增殖以及PWM驱动的IgG合成。抗1F 7抗体与约57%的未分级T细胞、62%的CD 4+细胞和54%的CD 8+细胞反应。尽管1F 7 Ag广泛分布于淋巴细胞中，但CD 4+细胞上的这种Ag优先在CDw 29（4 B4+）辅助细胞群上表达。此外，抗1F 7抗体进一步将CD 4 + CDw 29+细胞亚群细分为CDw 29 + 1F 7+和CDw 29 + 1F 7-群体。CD 4 + CDw 29 + 1F 7+细胞群最大限度地增殖以回忆Ag，如破伤风类毒素，而B细胞的PWM驱动的IgG合成的辅助功能属于CD 4 + CDw 29 + 1F 7+和CD 4 + CDw 29 + 1F 7-细胞群。该抗体定义的最突出的结构是110-kDa分子，其不同于抗CDw 29抗体鉴定的135-kDa、160-kDa和185-kDa糖蛋白以及UCHL-1抗体鉴定的180-kDa糖蛋白。然而，它与抗Ta 1识别的分子有关，抗Ta 1是T细胞上的活化Ag。此外，尽管Ta 1分子被抗1F 7 mAb识别，但1F 7结构家族还包括不同于Ta 1的分子。
We have developed a monoclonal antibody, anti-1F7, that inhibits soluble Ag-driven T cell proliferation as well as PWM-driven IgG synthesis. Anti-1F7 antibody reacts with approximately 57% of unfractionated T cells, 62% of CD4+ cells, and 54% of CD8+ cells. Although the 1F7 Ag is widely distributed among lymphoid cells, this Ag on CD4+ cells is preferentially expressed on the CDw29(4B4+) helper population. Moreover, anti-1F7 antibody further subdivides the CD4+CDw29+ cell subset into CDw29+1F7+ and CDw29+1F7- populations. The CD4+CDw29+1F7+ population of cells maximally proliferates to recall Ag such as tetanus toxoid, whereas helper function for PWM-driven IgG synthesis by B cells belongs to both the CD4+CDw29+1F7+ and CD4+CDw29+1F7- population of cells. The most prominent structure defined by this antibody is a 110-kDa molecule that is different from the 135-kDa, 160-kDa, and 185-kDa glycoproteins identified by anti-CDw29 antibody and the 180-kDa glycoprotein identified by UCHL-1 antibody. It is, however, related to the molecule recognized by anti-Ta1, an activation Ag on T cells. Furthermore, although the Ta1 molecule is recognized by anti-1F7 mAb, the 1F7 family of structures also includes molecules distinct from Ta1.