Major loss of junctional coupling during mitosis in early mouse embryos.
Major loss of junctional coupling during mitosis in early mouse embryos.
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DOI：
10.1083/jcb.102.2.568
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发表时间：
1986-02
期刊：
影响因子：
--
通讯作者：
Maro B
中科院分区：
文献类型：
--
作者：
Goodall H;Maro B
在小鼠胚胎发生的第四到第五细胞周期的过渡期间，通过注射羧基荧光素染料和测量细胞之间的电连续性来评估连接偶联。在早期8-细胞小鼠胚胎中从头开始的连接耦合，随后随着卵裂球进入有丝分裂而减少到细胞周期的末期。微管蛋白聚合的抑制剂诺可达唑使细胞周期停滞在中期，显示细胞在有丝分裂时的偶联变得不可检测。然后，在16-细胞期的间期恢复连接连接。诺可达唑本身对间期细胞的连接偶联没有影响，无论细胞间平坦化的程度如何，而作为微管稳定剂的紫杉醇确实减少了间期细胞的偶联程度。
Junctional coupling was assessed during the transition from the fourth to the fifth cell cycle of mouse embryogenesis by injection of the dye carboxyfluorescein and by measurement of electrical continuity between cells. Junctional coupling, which arises de novo in early 8-cell mouse embryos, subsequently becomes reduced towards the end of the cell cycle as the blastomeres enter into mitosis. Arrest of the cell cycle in metaphase by nocodazole, an inhibitor of tubulin polymerization, reveals that cell coupling becomes undetectable at mitosis. Junctional coupling then is resumed during interphase of the 16-cell stage. Nocodazole itself has no effect on junctional coupling in interphase cells, regardless of the extent of intercellular flattening, whereas taxol, a microtubule-stabilizing agent, does reduce the extent of coupling in interphase cells.