Coherent Raman scanning fiber endoscopy.
Coherent Raman scanning fiber endoscopy.
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DOI：
10.1364/ol.36.002396
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发表时间：
2011-07-01
期刊：
影响因子：
3.6
通讯作者：
Seibel EJ
中科院分区：
文献类型：
--
作者：
Saar BG;Johnston RS;Freudiger CW;Xie XS;Seibel EJ
相干拉曼散射方法允许具有化学对比度和高空间和时间分辨率的组织的无标记成像。然而，它们在散射组织中的成像深度被限制为小于1 mm，需要开发内窥镜以获得身体内部深处的图像。在这里，我们描述了一种相干拉曼内窥镜，其使用小型化光纤扫描仪、定制设计的物镜透镜和用于收集来自组织的散射光的优化方案以每秒七帧的速度提供受激拉曼散射图像。我们的系统的特点，并证明在小鼠组织图像的化学选择性。
Coherent Raman scattering methods allow for label-free imaging of tissue with chemical contrast and high spatial and temporal resolution. However, their imaging depth in scattering tissue is limited to less than 1 mm, requiring the development of endoscopes to obtain images deep inside the body. Here, we describe a coherent Raman endoscope that provides stimulated Raman scattering images at seven frames per second using a miniaturized fiber scanner, a custom-designed objective lens, and an optimized scheme for collection of scattered light from the tissue. We characterize the system and demonstrate chemical selectivity in mouse tissue images.