Isolation and characterization of CD8+ regulatory T cells in multiple sclerosis
Isolation and characterization of CD8+ regulatory T cells in multiple sclerosis
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DOI：
10.1016/j.jneuroim.2007.12.004
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发表时间：
2008-03-01
影响因子：
3.3
通讯作者：
Villa, Andres
中科院分区：
文献类型：
--
作者：
Correale, Jorge;Villa, Andres
为了研究CD 8+调节性T细胞对多发性硬化发展的影响，从20名急性加重期患者、15名缓解期患者和15名对照者中分离识别MBP 83 -102和MOG（63-87）特异性CD 4 + T细胞的外周血和脑脊液（CSF）CD 8 + T细胞克隆（TCC）。血液和CSF CD 8+调节性TCC克隆频率在急性加重期比缓解期或对照组下降更多。靶细胞预活化显著增强了CD 8 + T颗粒介导的对CD 4+靶细胞的杀伤，并受到HLA-E的限制。在急性加重期间，CD 8 + TCC中的肿瘤抑制受体CD 94/NKG 2A表达显著升高，限制了其细胞毒活性。此外，IL-15和IFN-γ显著增加CD 94和NKG 2A表达。这些数据提供了证据，证明CD 94/NKG 2A受体在MS过程中调节T细胞活性方面发挥重要作用。（C）2008 Elsevier B. V.保留所有权利。
To investigate CD8+ regulatory T cell influence on multiple sclerosis development, peripheral blood and cerebrospinal fluid (CSF) CD8+ T cell clones (TCCs) recognizing MBP83-102 and MOG(63-87)-specific CD4+ T cells were isolated from 20 patients during acute exacerbations, 15 in remission and 15 controls. Blood and CSF CD8+ regulatory TCC cloning frequency decreased more during exacerbations than remissions or controls. Target cell pre-activation significantly enhanced CD8+ T granule-mediated cell killing of CD4+ targets, and was restricted by HLA-E. During exacerbations, killer-inhibitory receptor CD94/NKG2A expression was significantly higher in CD8+ TCCs, limiting their cytotoxic activity. Moreover, IL-15 and IFN-gamma significantly increased CD94 and NKG2A expression. These data provide evidence that CD94/NKG2A receptors play an important role in regulating T cell activity during the course of MS. (C) 2008 Elsevier B.V. All rights reserved.