Long-lasting spontaneous calcium transients in the striatal cells
Long-lasting spontaneous calcium transients in the striatal cells
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DOI：
10.1016/j.neulet.2006.04.012
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发表时间：
2006-07
影响因子：
2.5
通讯作者：
M. Osanai;N. Yamada;T. Yagi
中科院分区：
文献类型：
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作者：
M. Osanai;N. Yamada;T. Yagi
纹状体在连接皮质活动和基底神经节输出方面起着重要作用。本研究采用急性脑片法，对纹状体的自发活动进行了钙（Ca ~（2+））显像研究。用Fura-PE_3-AM对大鼠脑皮质纹状体切片进行染色。观察到持续长达约250 s的长时间自发细胞内Ca 2+（[Ca 2 +]i）瞬变。瞬态的幅度是可变的，即使在一个单一的细胞。大多数细胞表现出不规则的频率，但有些表现出振荡的功能。这些[Ca ~（2+）] i传递不受河豚毒素的抑制，不受动作电位的诱导。离子型谷氨酸受体拮抗剂6-氰基-7-硝基喹喔啉-2，3-二酮和d，l-2-氨基-5-膦酰基戊酸不能阻断这些瞬变。这些结果提示，纹状体神经网络的动作电位和兴奋性突触输入不参与[Ca ~（2+）] i传递的诱导。与此相反，细胞内钙库消耗剂毒胡萝卜素（thapsigargin）大大减少了表现出[Ca 2 +]itransients的活性细胞的数量。因此，细胞内Ca ~（2+）库可能参与了[Ca ~（2+）] i的传递。
The striatum plays an important role in linking cortical activity to basal ganglia output. We conducted the calcium (Ca2+) imaging to investigate the spontaneous activities of the striatum using acute slice preparations. Corticostriatal slices of rat brain were stained with Fura-PE3-AM. Long-lasting spontaneous intracellular Ca2+([Ca2+]i) transients, which lasted up to about 250s, were observed. The amplitudes of the transients were variable even in a single cell. Most cells exhibited irregular frequencies, but some exhibited oscillatory features. These [Ca2+]itransients were not induced by action potentials because they were not inhibited by tetrodotoxin. Antagonists of the ionotropic glutamate receptors, 6-cyano-7-nitroquinoxaline-2,3-dione and d,l-2-amino-5-phosphonovaleric acid, did not block these transients. These results suggested that the action potentials and the excitatory synaptic inputs in these striatal network were not involved in the induction of the [Ca2+]itransients. In contrast, the number of the active cells, which exhibited the [Ca2+]itransients, was greatly reduced by the intracellular Ca2+store depletor, thapsigargin. Therefore, the intracellular Ca2+store is likely to contribute to the [Ca2+]itransients.