MACLURA-POMIFERA AGGLUTININ-BINDING GLYCOCONJUGATES ON CONVERTED APATITE FROM SYNTHETIC OCTACALCIUM PHOSPHATE IMPLANTED INTO SUBPERIOSTEAL REGION OF MOUSE CALVARIA
MACLURA-POMIFERA AGGLUTININ-BINDING GLYCOCONJUGATES ON CONVERTED APATITE FROM SYNTHETIC OCTACALCIUM PHOSPHATE IMPLANTED INTO SUBPERIOSTEAL REGION OF MOUSE CALVARIA
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DOI：
10.1016/s0169-6009(08)80024-4
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发表时间：
1993-02-01
期刊：
影响因子：
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通讯作者：
SAKURAI, M
中科院分区：
文献类型：
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作者：
SUZUKI, O;NAKAMURA, M;SAKURAI, M
我们之前已经证明，在小鼠颅骨骨膜下植入的合成磷酸钙（OCP）颗粒中的矿物质转化为磷灰石晶体，并且OCP植入刺激骨形成。在转化磷灰石上积累的基质成分与膜内成骨的骨结节（钙化起始点）的基质成分非常相似。在本研究中，我们比较了骨内OCP植入物与腹腔皮下植入物中基质的性质。通过使用已知对原膜内骨基质具有高亲和力的Maclura pomifera凝集素（MPA）凝集素进行比较。微束x射线衍射显示，10天后，植入的OCP在骨膜下和皮下部位就地转化为磷灰石晶体。在骨膜下植入13天后，在转化的磷灰石上检测到额外的骨形成，而在皮下植入中未形成骨。10天后，骨膜下和皮下植入的转化磷灰石基质中均出现了强烈的MPA反应。生化数据显示，所有小鼠血清、小鼠颅骨的胍基HCl-EDTA提取物和植入OCP的骨膜下和皮下部位的提取物中均存在强烈和弱mpa印迹分子（分别为53.0和152.6 kDa）。这些结果表明，OCP转化磷灰石上积累的糖缀合物与血清中的糖缀合物在对MPA和分子量的反应性方面相似。此外，结果表明，积累在转化磷灰石上的mpa结合糖缀合物可能是骨膜间充质细胞向成骨细胞分化的必要条件，而不是在皮下部位。
We have previously shown, that the mineral in granules of synthetic octacalcium phosphate (OCP) implanted subperiosteally in mouse calvariae was converted to apatitic crystals and that the OCP implantation stimulated bone formation. The matrix components accumulated on the converted apatite were very similar to those of bone nodules (starting locus of calcification) in intramembranous osteogenesis. In the present study, the nature of the matrices accumulated on OCP implants in calvariae was compared with that of the matrices accumulated in abdominal subcutaneous implants. The comparison was facilitated by the use of Maclura pomifera agglutinin (MPA) lectin which is known to have a high affinity for the primary intramembranous bone matrix. Micro-beam x-ray diffraction indicated conversion of the implanted OCP to apatitic crystals in situ, both in subperiosteal and subcutaneous sites, after 10 days. Additional bone formation was detected on the converted apatite after 13 days in subperiosteal implantation, whereas bone was not formed in the subcutaneous implantation. MPA reaction was strongly manifested after 10 days in matrices accumulated on the converted apatite in both subperiosteal and subcutaneous implantations. Biochemical data showed that intensely and weakly MPA-blotted molecules (53.0 and 152.6 kDa, respectively) were in all the mouse sera, in the guanidine HCl-EDTA extracts of mouse calvarial bone and in the extracts of the implanted OCP in both subperiosteal and subcutaneous sites. These findings indicated that the glycoconjugates accumulated on the converted apatite from OCP were similar to the glycoconjugates in the serum in terms of reactivity with MPA and molecular weights. Furthermore, the results suggest that MPA-binding glycoconjugates which had accumulated on the converted apatite may be a requisite for the differentiation of mesenchymal cells into osteoblasts in periosteum but not in subcutaneous sites.