HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ALPHA-KETO ACIDS IN PLASMA WITH FLUOROMETRIC DETECTION
HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF ALPHA-KETO ACIDS IN PLASMA WITH FLUOROMETRIC DETECTION
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DOI：
10.1016/s0378-4347(00)80946-9
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
NARUSE, H
中科院分区：
文献类型：
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作者：
HAYASHI, T;TSUCHIYA, H;NARUSE, H
一种用于定量测定α-β-环糊精的灵敏高效液相色谱方法，描述了使用荧光检测器测定[人]血浆中的酮酸。这个方法是. apprx。比以前的论文中报道的敏感10倍。仅需要50 μ l血浆来测定α-酮酸然而，对羟基苯基藜芦酸不能被分析，因为由其衍生的喹诺醇不显示荧光。
A sensitive high-performance liquid chromatographic method for the quantitative determination of .alpha.-keto acids in [human] plasma using a fluorescence detector is described. This method is .apprx. 10 times more sensitive than that reported in a previous paper. Only 50 .mu.l of plasma are needed for the determination of .alpha.-keto acids. However, p-hydroxyphenylpyruvic acid could not be analyzed because the quinoxalinol derived from it does not exhibit fluorescence.