Terminal phase of cytokinesis in D-98s cells.
Terminal phase of cytokinesis in D-98s cells.
复制标题
DOI：
10.1083/jcb.73.3.672
复制
发表时间：
1977-06
影响因子：
7.8
通讯作者：
Biesele, J J
中科院分区：
文献类型：
--
作者：
Mullins, J M;Biesele, J J
本文用光镜和电镜观察了D-98 S细胞在中间体和细胞间桥形成后胞质分裂完成的过程。从包埋的单层细胞中选择对应于细胞分裂素不同阶段的子细胞对进行连续切片。在分离子细胞之前，将细胞间桥的直径从1-1.5 μm减小到约1.5 μm。0.2μm。两个过程导致这种减少：（a）细胞间桥在末期后逐渐变薄;伴随着这种变化的是中间体结构的逐渐分解;（B）通过细胞间桥的一段局部收缩，对桥直径的减少做出更显著的贡献。桥的收缩部分内的微管被迫更紧密地靠在一起，并且随着这种变窄的进展，一些微管消失。在狭窄的部分上的质膜被抛成一系列波浪状的涟漪。子细胞的分离是通过细胞的运动来实现的，细胞的运动拉伸并破坏直径减小的桥。中间体在分离后被丢弃并开始变质。偶尔发现对子细胞，其中不完全的核分裂导致它们的细胞核由一条穿过桥和中体的核物质连接。这些细胞不完成胞质分裂，但在末期几小时后合并在一起。这种子细胞的合并与中间体几乎完全分解的过程相吻合。
The events leading to the completion of cytokinesis after the formation of the midbody and intercellular bridge in D-98S cells were studied with light and electron microscopy. Pairs of daughter cells corresponding to different stages of cytokineses, as determined previously form time lapse films, were selected from embedded monolayers for serial sectioning. Separation of daughter cells is preceded by the reduction in diameter of the intercellular bridge from 1-1.5 μm to approx. 0.2 μm. Two processes contribute to this reduction: (a) The intercellular bridge becomes gradually thinner after telophase; a progressive breakdown of midbody structures accompanies this change; and (b) the more significant contribution to reduction in bridge diameter occurs through the localized constriction of a segment of the intercellular bridge.. The microtubules within the constricted portion of the bridge are forced closer together, and some microtubules disappear as this narrowing progresses. The plasma membrane over the narrowed segments is thrown into a series of wavelike ripples. Separation of daughter cells is achieved through movements of the cells which stretch and break the diameter-reduced bridge. The midbody is discarded after separation and begins to deteriorate. Occasional pairs of daughter cells were found in which incomplete karyokineses resulted in their nuclei being connected by a strand of nuclear material traversing the bridge and midbody. Such cells do not complete cytokinesis but merge together several hours after telophase. This merging of daughter cells coincides with the nearly complete breakdown of the midbody.