MEX-5 and MEX-6 function to establish soma/germline asymmetry in early C-elegans embryos
MEX-5 and MEX-6 function to establish soma/germline asymmetry in early C-elegans embryos
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DOI：
10.1016/s1097-2765(00)80246-4
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发表时间：
2000-04-01
期刊：
影响因子：
16
通讯作者：
Priess, JR
中科院分区：
文献类型：
--
作者：
Schubert, CM;Lin, RL;Priess, JR
线虫卵受精后不久，由皮质定位的PAR蛋白组成的不对称网络形成。这一网络对于由非定位的、母性表达的mRNAs编码的几种蛋白质的表达模式的后续不对称是必需的。我们提供的证据表明，两个几乎相同的基因，MEX-5和MEX-6，将PAR不对称与随后的那些蛋白质不对称联系在一起。MEX-5是一种新的细胞质蛋白，通过PAR活性定位于1-细胞期胚胎的前极。MEX-5的定位与一组称为生殖系蛋白的后部定位蛋白的定位相反。MEX-5的异位表达足以抑制生殖系蛋白的表达，提示MEX-5具有抑制生殖系蛋白前向表达的功能。
An asymmetrical network of cortically localized PAR proteins forms shortly after fertilization of the C. elegans egg. This network is required for subsequent asymmetries in the expression patterns of several proteins that are encoded by nonlocalized, maternally expressed mRNAs. We provide evidence that two nearly identical genes, mex-5 and mex-6, link PAR asymmetry to those subsequent protein asymmetries. MEX-5 is a novel, cytoplasmic protein that is localized through PAR activities to the anterior pole of the 1-cell stage embryo. MEX-5 localization is reciprocal to that of a group of posterior-localized proteins called germline proteins. Ectopic expression of MEX-5 is sufficient to inhibit the expression of germline proteins, suggesting that MEX-5 functions to inhibit anterior expression of the germline proteins.