Use of biological detection methods to assess dioxin-like compounds in sediments of Bohai Bay, China
Use of biological detection methods to assess dioxin-like compounds in sediments of Bohai Bay, China
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利用生物检测方法评估中国渤海湾沉积物中的二恶英类化合物
DOI：
10.1016/j.ecoenv.2019.01.116
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发表时间：
2019
影响因子：
6.8
通讯作者：
Dong Wu
中科院分区：
文献类型：
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作者：
Dong Wenjing;Wang Feng;Fang Mingliang;Wu Jie;Wang Shuaiyu;Li Ming;Yang Jingfeng;Chernick Melissa;Hinton David E.;Pei De-Sheng;Chen Hongxing;Zheng Na;Mu Jingli;Xie Lingtian;Dong Wu
渤海湾位于中国东北渤海的西部地区，这里的水来自含有各种污染物的大河，包括类二恶英化合物（dlc）。本研究利用已建立的斑马鱼（Danio rerio）模型、已知的发育毒性终点和敏感分子分析对渤海湾某工业污水场附近及其周围的沉积物进行了评价。主要目的是评估快速生物检测方法作为化学分析的补充的功效。胚胎暴露于不同浓度的沉积物提取物和2,3,7,8 -四氯二苯并-对二恶英（TCDD）阳性对照。暴露于离排放地点最近的沉积物提取物（P1）导致胚胎和幼虫最严重和最高的变化率，这表明DLC污染的沉积物可能不会发生太多。P1提取物导致死亡率和心包水肿的浓度依赖性增加。其最高浓度导致受精后72 h (hpf) P-450 (CYP)-1A1(CYP1A) mRNA表达上调，全山原位杂交观察到其在鳃弓中的表达增加，并且在tg （CYP1A:mCherry）转基因系中信号增加。响应的模式和幅度与TCDD非常相似，支持dlc在这些沉积物样品中的存在。后续的化学分析证实了P1提取物中H7CDF、O8CDF和O8CDD为主要成分。本研究验证了生物测定法作为一种快速、灵敏、经济有效的方法来评估dlc及其在沉积物样品中的影响。此外，这也为dlc在海洋沉积物中的再动员能力有限的结论提供了支持。
Bohai Bay, in the western region of northeastern China's Bohai Sea, receives water from large rivers containing various pollutants including dioxin-like compounds (DLCs). This study used the established zebrafish (Danio rerio) model, its known developmental toxicity endpoints and sensitive molecular analyses to evaluate sediments near and around an industrial effluent site in Bohai Bay. The primary objective was to assess the efficacy of rapid biological detection methods as an addition to chemical analyses. Embryos were exposed to various concentrations of sediment extracts as well as a 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) positive control. Exposure to sediment extract nearest the discharge site (P1) resulted in the most severe- and highest rates of change in embryos and larvae, suggesting that DLC contaminated sediment probably did not occur much beyond it. P1 extract resulted in concentration dependent increases in mortality and pericardial edema. Its highest concentration caused up-regulation of P-450 (CYP)-1A1(CYP1A) mRNA expression at 72 h post fertilization (hpf), an increase in its expression in gill arches as observed by whole mountin situhybridization, and an increased signal in theTg(cyp1a:mCherry) transgenic line. The pattern and magnitude of response was very similar to that of TCDD and supported the presence of DLCs in these sediment samples. Follow-up chemical analysis confirmed this presence and identified H7CDF, O8CDF and O8CDD as the main components in P1 extract. This study validates the use of biological assays as a rapid, sensitive, and cost-effective method to evaluate DLCs and their effects in sediment samples. Additionally, it provides support for the conclusion that DLCs have limited remobilization capacity in marine sediments.