Electrical behavior of droplet interface bilayer networks: Experimental analysis and Modeling
Electrical behavior of droplet interface bilayer networks: Experimental analysis and Modeling
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DOI：
10.1021/ja074071a
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发表时间：
2007-09-26
影响因子：
15
通讯作者：
Bayley, Hagan
中科院分区：
文献类型：
--
作者：
Hwang, William L.;Holden, Matthew A.;Bayley, Hagan
浸没在油-脂质混合物中的水性液滴变得被脂质单层包围。液滴可以连接以形成具有诸如生物电池和光传感器的功能的液滴界面双层（DIB）的鲁棒网络。这样的DIB网络可以用作研究基于膜的生物现象的模型系统。在这项研究中，我们开发和实验验证了DIB网络的电建模方法，通过应用它来描述通过一个简单的网络包含蛋白质孔和阻断分子的电流。我们演示了使用SPICE（模拟程序与集成电路强调）模拟DIB网络的电气行为。DIB网络的模块化和可扩展性，使本文提出的分析，以大型，复杂的网络直接扩展。
Aqueous droplets submerged in an oil-lipid mixture become enclosed by a lipid monolayer. The droplets can be connected to form robust networks of droplet interface bilayers (DIBs) with functions such as a biobattery and a light sensor. Such DIB networks might be used as model systems for the study of membrane-based biological phenomena. In this study, we develop and experimentally validate an electrical modeling approach for DIB networks by applying it to describe the current flow through a simple network containing protein pores and blocking molecules. We demonstrate the use of SPICE (Simulation Program with Integrated Circuit Emphasis) for simulating the electrical behavior of DIB networks. The modular and scalable nature of DIB networks should enable a straightforward extension of the analysis presented in this paper to large, complex networks.