The human tissue plasminogen activator-Cre mouse: a new tool for targeting specifically neural crest cells and their derivatives in vivo
The human tissue plasminogen activator-Cre mouse: a new tool for targeting specifically neural crest cells and their derivatives in vivo
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DOI：
10.1016/s0012-1606(03)00175-1
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发表时间：
2003-07-01
影响因子：
2.7
通讯作者：
Dufour, S
中科院分区：
文献类型：
--
作者：
Pietri, T;Eder, O;Dufour, S
神经嵴细胞（NCC）的个体发生涉及许多协调的各种衍生物，包括外周神经系统和黑素细胞的成分。因此，它代表了一个很好的模型系统，研究控制上皮-间充质转化，细胞迁移和分化，以及细胞增殖和死亡的机制。我们已经建立了一个新的转基因株系表达Cre重组酶的控制下的人组织纤溶酶原激活剂启动子（Ht-PA）。早在头间充质中的Theiler阶段12就观察到Ht-PA-Cre/R26 R胚胎中报告基因的活性。后来，靶细胞包括所有已知的颅、迷走神经和躯干NCC的衍生物，包括颅面神经。结构和颅神经节、心脏和内分泌衍生物、黑素细胞、外周和肠神经系统。在迷走神经水平，推定的肠NCC的位置不同，从他们的鸟类同行的能力，侵入间充质侧的神经管。与Wnt 1-Cre系相反，Ht-PA-Cre系不靶向中枢神经系统，因此使其对NCC更具特异性。我们的Ht-PA-Cre小鼠代表了一种特异性靶向迁移性NCC中条件突变的新型模型。(C)2003 Elsevier Science（美国）。All rights reserved.
The ontogeny of neural crest cells (NCC) involves a number of orchestrated variety of derivatives, including components of the peripheral nervous system and melanocytes. Thus, it represents an excellent model system to investigate mechanisms controlling epithelial-mesenchymal transitions, cell migration and differentiation, as well as cell proliferation and death. We have established a new transgenic line expressing the Cre recombinase under the control of the human tissue plasminogen activator promoter (Ht-PA). The activity of the reporter in the Ht-PA-Cre/R26R embryos is observed as early as Theiler stage 12 in the cephalic mesenchyme. Later, the targeted cells include all the known derivatives of cranial, vagal, and trunk NCC, including craniofacial. structures and cranial ganglia, cardiac and endocrine derivatives, melanocytes, peripheral, and enteric nervous system. At the vagal level, the location of presumptive enteric NCC differs from their avian counterparts in their ability to invade the mesenchyme lateral to the neural tube. In contrast to the Wnt1-Cre line, the Ht-PA-Cre line does not target the central nervous system and therefore renders it more specific for NCC. Our Ht-PA-Cre mice represent a novel model to specifically target conditional mutations in migratory NCC. (C) 2003 Elsevier Science (USA). All rights reserved.