Microinjected DNA from the X chromosome affects sex determination in Caenorhabditis elegans.
Microinjected DNA from the X chromosome affects sex determination in Caenorhabditis elegans.
复制标题
显微注射 X 染色体 DNA 会影响秀丽隐杆线虫的性别决定。
DOI：
10.1126/science.2973125
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Meneely,PM
中科院分区：
文献类型：
--
作者：
McCoubrey,WK;Nordstrom,KD;Meneely,PM
线虫秀丽隐杆线虫性别决定信号是 X 染色体数量与常染色体组数的比率（X/A 比率）。通过之前的基因测试，使染色体男性女性化的元素似乎广泛存在于X染色体上，但这些元素的性质尚未确定。在从分子角度定义女性化元素的实验中，通过显微注射将克隆序列添加到染色体男性胚胎中。三个不同的 X 染色体克隆，包括肌动蛋白基因的一部分、肌球蛋白重链基因的一部分和所有两个肌球蛋白轻链基因，使染色体男性女性化。性别决定的体细胞和种系方面都受到影响。相比之下，约 40 kb 的线虫常染色体 DNA、噬菌体 lambda DNA 和质粒 pBR322 DNA 不会影响性别决定。女性化区域位于 X 连锁肌动蛋白基因内含子内最多 131 个碱基对；没有该区域的基因部分不是女性化的。结果表明，与许多 X 连锁基因相关的短而离散的元件可能作为 C 性别决定的信号。线虫。
The signal for sex determination in the nematodeCaenorhabditis elegansis the ratio of the number of X chromosomes to the number of sets of autosomes (X/A ratio). By previous genetic tests, elements that feminized chromosomal males appeared to be widespread on the X chromosome, but the nature of these elements was not determined. In experiments to define a feminizing element molecularly, cloned sequences were added to chromosomally male embryos by microinjection into the mother. Three different X-chromosome clones, including part of an actin gene, part of a myosin heavy chain gene, and all of two myosin light chain genes, feminize chromosomal males. Both somatic and germline aspects of sex determination are affected. In contrast, about 40 kilobases of nematode autosomal DNA, phage lambda DNA, and plasmid pBR322 DNA do not affect sex determination. A feminizing region was localized to a maximum of 131 base pairs within an intron of the X-linked actin gene; a part of the gene that does not have this region is not feminizing. The results suggest that short, discrete elements found associated with many X-linked genes may act as signals for sex determination inC. elegans.