ANALYSIS OF PHYTOPLANKTON PIGMENTS BY EXCITATION SPECTRA OF FLUORESCENCE
ANALYSIS OF PHYTOPLANKTON PIGMENTS BY EXCITATION SPECTRA OF FLUORESCENCE
复制标题
DOI：
--
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
L. Poryvkina;S. Babichenko;A. Leeben
中科院分区：
文献类型：
--
作者：
L. Poryvkina;S. Babichenko;A. Leeben
讨论了主动激发荧光法在天然浮游植物分析中应用的优势和存在的问题。重点是通过荧光数据正确预测颜料浓度。给出了1997年和1998年戈特兰盆地(波罗的海)蓝藻水华期间的叶绿素和藻胆蛋白的高分辨率测绘结果。浮游植物色素的动态空间图与流动光谱荧光成像仪和舰载激光遥感光谱仪FLS-S在线配准。讨论了研究区浮游植物的分布特征及其随时间的演变。现场研究证实，在浮游植物水华检测、浮游植物生长发育分析和光合作用色素空间测绘等任务中，在线荧光光谱仪可以有效地用于浮游植物色素的诊断。考虑到监测大面积水域或现场临时过程的任务，最有成效的方法是在线连续荧光测量和采样程序的平衡组合，这允许减少实验室对水样进行耗时的人工分析。
The advantages and problems of the application of actively excited fluorescence in natural phytoplankton analysis are discussed. The focus is made to a correct prediction of pigment concentrations by fluorescence data. The results of high resolution mapping of chlorophylls and phycobilins in the Gotland Basin (Baltic Sea) during Cyanobacterial blooms in 1997 and 1998 are presented. Dynamical spatial maps of phytoplankton pigments were registered on line with the flow-through spectrofluorimeter FLUO-IMAGER and the shipboard laser remote sensing spectrometer FLS-S. Characteristic patterns of the phytoplankton distribution in the study area and their evolution in time are discussed. Field studies confirmed that on line spectrofluorimetry can be effectively used to diagnose phytoplankton pigments in application to the tasks of phytoplankton bloom detection, analysis of phytoplankton development and spatial mapping of photosyntheti c pigments. Considering the tasks of monitoring large water areas or temporary processes in a spot area, the most productive way is a balanced combination of on line continuous fluorescence measurements and sampling procedures, which allows to decrease the time-consuming manual analysis of water samples in the laboratory.