Effects of dietary omega-3 fatty acids on ventricular function in dogs with healed myocardial infarctions: in vivo and in vitro studies
Effects of dietary omega-3 fatty acids on ventricular function in dogs with healed myocardial infarctions: in vivo and in vitro studies
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DOI：
10.1152/ajpheart.01065.2009
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发表时间：
2010-04-01
影响因子：
4.8
通讯作者：
Janssen, Paul M. L.
中科院分区：
文献类型：
--
作者：
Billman, George E.;Nishijima, Yoshinori;Janssen, Paul M. L.
Billman GE, Nishijima Y, Belevych AE, Terentyev D, Xu Y, Haizlip KM, Monasky MM, Hiranandani N, Harris WS, Gyorke S, Carnes CA, Janssen PM。膳食omega-3脂肪酸对心肌梗死愈合犬心室功能的影响：体内和体外研究[J]中国生物医学工程学报，2016,31(2):557 - 557。首次发表于2010年1月22日；doi: 10.1152 / ajpheart.01065.2009。由于omega-3多不饱和脂肪酸（n-3 PUFAs）可以改变心室肌细胞对钙的处理，这些脂肪酸可能对心脏收缩功能产生不利影响，特别是在心肌梗死后。因此，心肌梗死后4周，狗被随机分配给安慰剂（玉米油，1 g/天，n = 16）或n-3 PUFAs补充剂[二十二碳六烯酸(DHA) +二十碳五烯酸（EPA）乙酯；1、2或4克/天；N = 7、8、12]组。在体内，治疗前后3个月通过超声心动图评估心室功能。3个月后，取出心脏，用右心室小梁和分离的左心室肌细胞评估其体外功能。治疗引起左心室组织和红细胞n-3 PUFA水平显著（P < 0.0001）剂量依赖性增加（4 g/天增加16.4倍）（EPA + DHA，安慰剂，0.42 +/- 0.04；1 g/天，3.02 +/- 0.23；2 g/天，3.63 +/- 0.17；4 g/天，6.97 +/- 0.33%）。无论剂量如何，n-3 PUFA治疗并未改变完整动物的心室功能（例如，4 g/天，部分缩短：前，42.9 +/- 1.6 vs后，40.1 +/- 1.7%；安慰剂：前，39.2 +/- 1.3 vs后，38.4 +/- 1.6%）。每横截面积的发展力，收缩力的长度和频率依赖行为的变化，以及对β -肾上腺素能素激活的肌力反应，在安慰剂或n-3 pufa治疗的狗小梁中也相似。最后，在n-3 PUFA和安慰剂治疗的狗的肌细胞中，钙电流和钙瞬态是相同的。因此，饮食中的n-3 PUFAs无论在体外还是体内都不会对梗死愈合犬的心室收缩功能产生不利影响。
Billman GE, Nishijima Y, Belevych AE, Terentyev D, Xu Y, Haizlip KM, Monasky MM, Hiranandani N, Harris WS, Gyorke S, Carnes CA, Janssen PM. Effects of dietary omega-3 fatty acids on ventricular function in dogs with healed myocardial infarctions: in vivo and in vitro studies. Am J Physiol Heart Circ Physiol 298: H1219-H1228, 2010. First published January 22, 2010; doi: 10.1152/ajpheart.01065.2009.-Since omega-3 polyunsaturated fatty acids (n-3 PUFAs) can alter ventricular myocyte calcium handling, these fatty acids could adversely affect cardiac contractile function, particularly following myocardial infarction. Therefore, 4 wk after myocardial infarction, dogs were randomly assigned to either placebo (corn oil, 1 g/day, n = 16) or n-3 PUFAs supplement [ docosahexaenoic acid (DHA) + eicosapentaenoic acid (EPA) ethyl esters; 1, 2, or 4 g/day; n = 7, 8, and 12, respectively] groups. In vivo, ventricular function was evaluated by echocardiography before and after 3 mo of treatment. At the end of the 3-mo period, hearts were removed and in vitro function was evaluated using right ventricular trabeculae and isolated left ventricular myocytes. The treatment elicited significant (P < 0.0001) dose-dependent increases (16.4-fold increase with 4 g/day) in left ventricular tissue and red blood cell n-3 PUFA levels (EPA + DHA, placebo, 0.42 +/- 0.04; 1 g/day, 3.02 +/- 0.23; 2 g/day, 3.63 +/- 0.17; and 4 g/day, 6.97 +/- 0.33%). Regardless of the dose, n-3 PUFA treatment did not alter ventricular function in the intact animal (e.g., 4 g/day, fractional shortening: pre, 42.9 +/- 1.6 vs. post, 40.1 +/- 1.7%; placebo: pre, 39.2 +/- 1.3 vs. post, 38.4 +/- 1.6%). The developed force per cross-sectional area, changes in length- and frequency-dependent behavior in contractile force, and the inotropic response to beta-adrenoceptor activation were also similar for trabeculae obtained from placebo- or n-3 PUFA-treated dogs. Finally, calcium currents and calcium transients were the same in myocytes from n-3 PUFA- and placebo-treated dogs. Thus dietary n-3 PUFAs did not adversely alter either in vitro or in vivo ventricular contractile function in dogs with healed infarctions.