A low-protein isocaloric diet during gestation affects brain development and alters permanently cerebral cortex blood vessels in rat offspring
A low-protein isocaloric diet during gestation affects brain development and alters permanently cerebral cortex blood vessels in rat offspring
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DOI：
10.1093/jn/129.8.1613
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发表时间：
1999-08-01
影响因子：
4.2
通讯作者：
Reusens, B
中科院分区：
文献类型：
--
作者：
Bennis-Taleb, N;Remacle, C;Reusens, B
在人类中，低出生体重与成年后的非致命性中风、心血管疾病和糖尿病有关。本研究的目的是在大鼠中研究早期蛋白质限制，诱导低出生体重，对出生时大脑和内分泌胰腺血管化的影响，并研究这种改变是否持续到成年。给怀孕的大鼠喂食20%或8%的蛋白质等热量饮食。对照组幼崽由饲粮蛋白质含量为20%的母鼠喂养，低蛋白（LP）幼崽由饲粮蛋白质含量为8%或20%的母鼠喂养。哺乳期间的饮食一直维持到成年。用形态测定法分析，饲喂8%蛋白质日粮的3龄幼崽大脑皮层血管密度低于对照组(C)。在成年后，无论是低脂饮食还是低脂饮食，它都保持较低水平。由于朗格汉斯胰岛的血管化在新生儿中减少，但在出生后通过C饮食在成年后恢复正常，因此限制在胎儿期的低脂饮食诱导的成年期血管化减少似乎是大脑的特征。LP幼犬和成年犬的体重和脑重较低。LP幼犬前脑DNA浓度较低，小脑DNA浓度较高。LP成人大脑DNA、蛋白质、胆固醇和磷脂浓度较高，出生后通过正常饮食恢复到成年期。总之，在胎儿发育期间暴露于低热量等热量饮食的后代的大脑皮层显示出血管化减少，并持续一生。
In humans, low birth weight is associated with nonfatal stroke, cardiovascular disease and diabetes at adulthood. The aim of this study was to investigate in rats the effect of early protein restriction, inducing low birth weight, on brain and endocrine pancreas vascularization at birth and to study if such alterations lasted until adulthood. Pregnant rats were fed either 20 or 8% protein isocaloric diets. Control newborns were nursed by their dams fed the 20% protein diet and low protein (LP) pups by dams fed either the 8 or 20% protein diet. The diets given during lactation were maintained until adulthood. The blood vessel density of cerebral cortex analyzed by morphometry in 3-d-old pups from dams fed the 8% protein diet was lower than in control (C). It remained lower at adulthood whether a LP or a C diet was given postnatally. Reduction of vascularization at adulthood induced by the LP diet limited to fetal life seems characteristic for the brain since vascularization of islets of Langerhans was reduced in neonates but normalized at adulthood by a C diet postnatally. Body and brain weights were lower in LP pups and adults. DNA concentration was lower in forebrain and higher in cerebellum in LP pups. In brain of LP adults, DNA, protein, cholesterol and phospholipid concentrations were tower and were restored at adulthood by a normal diet after birth. In conclusion, cerebral cortex of offspring exposed to a LP isocaloric diet during fetal development showed reduced vascularization which remained throughout life.