A review of bone cell and substratum interactions: An illustration of the role of scanning electron microscopy
A review of bone cell and substratum interactions: An illustration of the role of scanning electron microscopy
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骨细胞和基质相互作用的回顾：扫描电子显微镜作用的例证
DOI：
10.1002/sca.4950070101
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
E. Maconnachie
中科院分区：
文献类型：
--
作者：
Sheila J. Jones;A. Boyde;N. Ali;E. Maconnachie
关于观察骨细胞功能形态变化的第一个实验研究是使用SEM进行的，并且SEM在该领域中仍然是最重要的。骨形成和再吸收细胞仅存在于表面上-去除相邻组织层后可用于研究：然后可以在去除细胞后研究底层基质表面，并在去除基质后检查矿物质前沿（使用适当的溶剂或通过等离子体灰化）。
在这篇综述中，我们分析了我们在这个实验室中所取得的关于成骨细胞（骨形成细胞）和破骨细胞（骨吸收细胞）的生物活性的主要发现。细胞比其所附着的基质收缩更多，其标本制备的技术问题以前已被讨论过（Boyde等，1977）。这样的问题显然影响横向，细胞与细胞的相互关系比细胞基板的影响，我们在这里覆盖。
目前，我们可以得出这样的结论，扫描电镜已作出了重大贡献，骨生物学允许观察正常细胞和天然和替代基板。我们有信心地预测，它将继续发挥关键作用，在更密切地观察细胞-细胞-基质相互作用，特别是在局部激素的影响，以及在骨病理学和种植。
The first experimental studies concerning observations of changes in bone cell functional morphology were made using the SEM, and SEM has remained paramount in this field. Bone forming and resorbing cells only exist on surfaces – which are available for study after removal of adjacent tissue layers: The underlying matrix surface can then be studied after removal of the cells, and the mineral front examined after removing the matrix (with an appropriate solvent or by plasma ashing).
In this review, we analyse the main findings which we have made in this laboratory concerning the biological activities of osteoblasts (bone forming cells) and osteoclasts (bone resorbing cells). The technical problems of specimen preparation of cells which shrink more than the substrate to which they are attached have been convered previously (Boyde et al. 1977). Such problems obviously affect the lateral, cell to cell inter-relationships more than the cell to substrate effects which we cover here.
At present, we can conclude that SEM has made a major contribution to bone biology by permitting observation of normal cells and natural and surrogate substrates. We confidently predict that it will continue to play a pivotal role in the closer observation of cell-cell-substrate interactions particularly in respect of local hormonal effects, as well as in bone pathology and implantology.