Multiple pathways of mitochondrial-nuclear communication in yeast: intergenomic signaling involves ABF1 and affects a different set of genes than retrograde regulation.
Multiple pathways of mitochondrial-nuclear communication in yeast: intergenomic signaling involves ABF1 and affects a different set of genes than retrograde regulation.
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DOI：
10.1016/j.bbagrm.2008.09.008
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发表时间：
2009-02
期刊：
影响因子：
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通讯作者：
D. Woo;Tzulip Phang;J. Trawick;R. O. Poyton
中科院分区：
文献类型：
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作者：
D. Woo;Tzulip Phang;J. Trawick;R. O. Poyton
线粒体-核通讯在氧传感、氧化应激、衰老和疾病的模型中越来越重要。线粒体基因组（mtDNA）的缺失以及因此呼吸能力的缺失，通过至少两种不同的线粒体-核通讯途径影响几种核基因的表达。其中一条途径，逆行调节，是由呼吸减少激活的，而另一条途径，基因组间信号传导，不受呼吸影响，但需要mtDNA。使用DNA微阵列，我们在这里确定了一组核基因在酿酒酵母基因组间信号的目标。这些核基因在缺乏mtDNA的rho细胞中下调，但在具有mtDNA但缺乏呼吸的核pet突变rho+细胞中不下调。这些核基因中的许多编码线粒体蛋白，这意味着基因组间信号传导在协调线粒体和核基因表达中起作用。此外，COX 6基因（一种受基因组间信号传导影响的核基因）启动子中的缺失和接头扫描突变分析表明Abf 1 p转录因子参与基因组间信号传导。总之，这些发现表明，基因组间信号传导与逆行调节不同，无论是在它调节的核基因中，还是在它影响它们表达的方式中。
Mitochondrial-nuclear communication is taking on increased importance in models of oxygen sensing, oxidative stress, aging, and disease. The deletion of the mitochondrial genome (mtDNA) and, hence, the ability to respire, affects expression of several nuclear genes through at least two different mitochondrial-nuclear communication pathways. One of the pathways, retrograde regulation, is activated by a reduction in respiration, while another, intergenomic signaling, is unaffected by respiration but requires mtDNA. Using DNA microarrays, we identify here a set of nuclear genes in Saccharomyces cerevisiae that are targets of intergenomic signaling. These nuclear genes are down-regulated in rho° cells that lack mtDNA but not in nuclear pet mutant rho+cells that possess mtDNA but lack respiration. Many of these nuclear genes encode mitochondrial proteins, implying that intergenomic signaling functions in coordinating mitochondrial and nuclear gene expression. In addition, analyses of deletion and linker scanning mutations in the promoter of the COX6 gene, a nuclear gene affected by intergenomic signaling, suggest an involvement of Abf1p transcription factor in intergenomic signaling. Together, these findings indicate that intergenomic signaling is distinct from retrograde regulation both in the nuclear genes that it regulates and in the way in which it affects their expression.