Measurement of Predation and Biofilm Formation under Different Ambient Oxygen Conditions Using a Simple Gasbag-Based System
Measurement of Predation and Biofilm Formation under Different Ambient Oxygen Conditions Using a Simple Gasbag-Based System
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DOI：
10.1128/aem.01193-13
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发表时间：
2013-09-01
影响因子：
4.4
通讯作者：
Tran, Aimy
中科院分区：
文献类型：
--
作者：
Kadouri, Daniel E.;Tran, Aimy
噬菌蛭弧菌（Bdellovibrio bacteriovorus）和嗜水气单胞菌（Micavibrio aerodynosavorus）是革兰氏阴性菌，具有捕食性行为。本研究的目的是评估捕食者在不同氧气环境中的捕食能力。当放置在一个定轨振荡器，通气率和捕食率之间的正相关进行了观察。为了进一步研究环境氧水平升高对捕食的影响，开发了一个简单的气囊系统。使用该系统，我们能够在3%至86%的环境氧气水平下进行实验。当放置在气囊和充气，50%O-2，和100%O-2，积极的捕食被认为是对这两个反硝化和生物膜生长的猎物细胞。然而，在低氧环境中，捕食性细菌只能攻击作为生物膜生长的猎物细胞。为了进一步评估气囊系统，还测量了革兰氏阳性和革兰氏阴性微生物的生物膜发展。尽管发现气囊系统适合于培养需要低氧环境的细菌，但其当前配置不能支持专性厌氧菌在液体或琼脂培养基中的生长。
Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are Gram-negative bacteria characterized by predatory behavior. The aim of this study was to evaluate the ability of the predators to prey in different oxygen environments. When placed on an orbital shaker, a positive association between the rate of aeration and predation was observed. To further examine the effects of elevated ambient oxygen levels on predation, a simple gasbag system was developed. Using the system, we were able to conduct experiments at ambient oxygen levels of 3% to 86%. When placed in gasbags and inflated with air, 50% O-2, and 100% O-2, positive predation was seen on both planktonic and biofilm-grown prey cells. However, in low-oxygen environments, predatory bacteria were able to attack only prey cells grown as biofilms. To further evaluate the gasbag system, biofilm development of Gram-positive and Gram-negative microorganisms was also measured. Although the gasbag system was found to be suitable for culturing bacteria that require a low-oxygen environment, it was not capable of supporting, with its current configuration, the growth of obligate anaerobes in liquid or agar medium.