Culture-independent diagnosis of Chlamydia trachomatis using monoclonal antibodies.
Culture-independent diagnosis of Chlamydia trachomatis using monoclonal antibodies.
复制标题
使用单克隆抗体对沙眼衣原体进行不依赖培养的诊断。
DOI：
10.1056/nejm198405033101803
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Nowinski,RC
中科院分区：
文献类型：
--
作者：
Tam,MR;Stamm,WE;Handsfield,HH;Stephens,R;Kuo,CC;Holmes,KK;Ditzenberger,K;Krieger,M;Nowinski,RC
为了简化衣原体生殖器感染的诊断，我们使用荧光素偶联单克隆抗体对直接从患者尿道或宫颈分泌物制备的涂片进行免疫荧光试验。这种直接测试，需要不到30分钟的时间来执行，是基于检测细胞外衣原体初级体。对926名患者标本进行的直接试验与碘染色培养物的比较表明，灵敏度为93%，特异性为96%。直接试验为衣原体感染的诊断提供了一种快速、简单和敏感的方法，可在不具备组织培养能力的实验室中进行。（中华医学杂志1984；31:1146 - 50）
To simplify the diagnosis of chlamydial genital infection, we used a fluorescein-conjugated monoclonal antibody in immunofluorescence tests on smears prepared from urethral or cervical secretions obtained directly from patients. This direct test, requiring less than 30 minutes to perform, was based on the detection of extracellular chlamydial elementary bodies. A comparison of the direct test with cultures stained with iodine on specimens from 926 patients demonstrated a sensitivity of 93 per cent and a specificity of 96 per cent. The direct test provides a rapid, simple, and sensitive method for the diagnosis of chlamydial infection, which can be performed in laboratories that do not have tissue-culture capability. (N Engl J Med 1984; 310:1146–50.)