Three-dimensional imaging of tumor angiogenesis.
Three-dimensional imaging of tumor angiogenesis.
复制标题
肿瘤血管生成的三维成像。
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
R. Osamura
中科院分区：
文献类型：
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作者：
J. Itoh;K. Yasumura;T. Takeshita;H. Ishikawa;H. Kobayashi;K. Ogawa;Kenji Kawai;A. Serizawa;R. Osamura
客观的
OBJECTIVE
To three-dimensionally visualize the microvessel environment of tumor angiogenesis by confocal laser scanning microscopy (CLSM).
STUDY DESIGN
To reveal underlying mechanisms of tumor angiogenesis, a 7, 12-dimethylbenz(a) anthracene-induced rat cancer model was used. For demonstrating tumor vasculature, fluorescence injection method (FITC-conjugated gelatin solution) was employed. FITC gelatin was injected into the left ventricle of the rat heart. After complete perfusion, the mammary glands were resected, fixed under ice cold conditions and subjected to immunohistochemistry (IHC) for tumor cells. The LSM-410 (Carl Zeiss, Jena, Germany) was employed on thick sections (300-2,000 microns) to elucidate detailed microvessel networks (MVN) and tumor cells.
RESULTS
Tumor vasculature on thick sections was clearly detected by CLSM at the maximum focus depth of 2,000 microns. Three-dimensional (3-D), reconstructed images of normal mammary glands showed regular and linear MVN. In DMBA-induced mammary cancer, vascular density of MVN was markedly increased and showed an anastomosing, irregular MVN pattern. Furthermore, focal segmentation and tortuous, branching patterns of microvessels were also seen.
CONCLUSION
Application of the fluorescence injection method and IHC using CLSM was very useful for studying the 3-D relationship between tumor angiogenesis and neoplastic epithelial changes. These results suggest that application of this technique is ideal for studying 3-D imaging of tumor angiogenesis.