EXPOSURE OF AN ANTIGEN OF CHROMAFFIN GRANULES ON CELL-SURFACE DURING EXOCYTOSIS
EXPOSURE OF AN ANTIGEN OF CHROMAFFIN GRANULES ON CELL-SURFACE DURING EXOCYTOSIS
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DOI：
10.1038/301610a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
WINKLER, H
中科院分区：
文献类型：
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作者：
LINGG, G;FISCHERCOLBRIE, R;WINKLER, H
肾上腺髓质儿茶酚胺储存囊泡（染色质颗粒）膜蛋白的合成速率低于其分泌蛋白的合成速率1。基于这些结果，我们提出2,3，在胞吐后，嗜铬颗粒的膜被回收，并在几个分泌周期中重复使用（另见参考文献4）。在刺激过程中被分泌细胞吸收的外源标记物可以追溯到高尔基区和未成熟的分泌细胞器5,6，这一发现进一步加强了颗粒膜再利用的概念。然而，一个基本问题仍然存在：分泌细胞器的膜是否特异性地和完全地从质膜上移除，如果是，这个过程有多快？通过使用抗血清对抗染色质颗粒的膜糖蛋白，我们现在已经获得了定量数据，证明在胞吐过程中，这种抗原暴露在细胞表面，并在30分钟内再次大量消失。
The synthesis rate of the membrane proteins of the catecholamine-storing vesicles (chromaffin granules) of the adrenal medulla is lower than that of the secretory proteins of the contents1. Based on these results we proposed2,3that after exocytosis the membranes of chromaffin granules are retrieved and are re-used for several secretion cycles (see also ref. 4). This concept of re-use of granule membranes has been further strengthened by the finding that exogenous markers which are taken up by secretory cells during stimulation can be traced to the Golgi region and to immature secretory organelles5,6. However, one basic question remains: are the membranes of secretory organelles specifically and completely removed from the plasma membrane and if so, how fast is this process? By using an antiserum against a membrane glycoprotein of chromaffin granules we have now obtained quantitative data which demonstrate that during exocytosis this antigen becomes exposed on the cell surface and disappears again to a large degree within 30 min.