Pathway‐Selection for Programmable Assembly of Genetically Encoded Amphiphiles by Thermal Processing
Pathway‐Selection for Programmable Assembly of Genetically Encoded Amphiphiles by Thermal Processing
复制标题
通过热处理对基因编码两亲物进行可编程组装的途径选择
DOI：
10.1002/syst.202100037
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Mozhdehi, Davoud
中科院分区：
文献类型：
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作者：
Khodaverdi, Masoumeh;Hossain, Md Shahadat;Zhang, Zhe;Martino, Robert P.;Nehls, Connor W.;Mozhdehi, Davoud
通过热处理(即 ，使用热制备路径)来设计超分子组装体的命运仍然具有挑战性，并且在很大程度上是一个临时过程。这种轨迹的合理设计需要沿着自由能随温度变化而定义的组装路径精确地操纵分子加密相互作用的强度。在这里，我们使用温度触发的蛋白质液-液相分离来编程，沿着用户定义的热轨迹，对基因编码的两亲分子--一种翻译后脂化的蛋白质--进行纳米和中尺度组装。这种分子定义的途径被用来将热能输送到系统中，将构建块引导到以前无法到达的能量景观区域，并合成具有复杂结构的二维纳米材料，如辫子和环状结构。由于蛋白质的相界和转变是在序列水平上可编程的，我们的策略开辟了新的视野，以扩展蛋白质材料的结构层次和功能格局。
Engineering the fate of supramolecular assemblies by heat treatment (i. e., using thermal preparative pathways) remains challenging and a largely ad hoc process. The rational design of such trajectories requires precise manipulation of the strength of molecularly encrypted interactions along an assembly path defined by changes in free energy as a function of temperature. Here, we use temperature‐triggered liquid–liquid phase separation of proteins to program the nano‐ and mesoscale assembly of a genetically encoded amphiphile – a post‐translationally lipidated protein – along user‐defined thermal trajectories. This molecularly defined pathway was used to funnel thermal energy into the system to direct building blocks to a previously inaccessible region of the energy landscape, and to synthesize two‐dimensional nanomaterials with sophisticated structures, such as braids and toroids. Because proteins’ phase boundaries and transition are programmable at the sequence‐level, our strategy opens new horizons to expand the structural hierarchy and functional landscape of protein‐based materials.