The Assembly Line Enzymology of Polyketide Biosynthesis.
The Assembly Line Enzymology of Polyketide Biosynthesis.
复制标题
DOI：
10.1007/978-1-4939-3375-4_2
复制
发表时间：
2016
影响因子：
--
通讯作者：
M. Till;P. Race
中科院分区：
文献类型：
--
作者：
M. Till;P. Race
聚酮化合物是一类具有结构和功能多样性的天然生物活性物质，在医药、农药和兽药等领域有着广泛的应用。在细菌中，复杂的聚酮化合物由称为模块化聚酮化合物酶（PKS）的巨型多功能巨酶生物合成，所述模块化聚酮化合物酶以高度协调的装配线样方式从简单的前体底物池构建其产物。PKSs的多方面酶学不仅是一个迷人的研究目标，但它也提供了相当大的机会，这些系统的再造，提供获得功能优化的非天然天然产物。在这里，我们提供了一个介绍性的引物模块化聚酮合酶的结构和功能，并强调这些系统的表征和开发的最新进展。
Polyketides are a structurally and functionally diverse family of bioactive natural products that have found widespread application as pharmaceuticals, agrochemicals, and veterinary medicines. In bacteria complex polyketides are biosynthesized by giant multifunctional megaenzymes, termed modular polyketide synthases (PKSs), which construct their products in a highly coordinated assembly line-like fashion from a pool of simple precursor substrates. Not only is the multifaceted enzymology of PKSs a fascinating target for study, but it also presents considerable opportunities for the reengineering of these systems affording access to functionally optimized unnatural natural products. Here we provide an introductory primer to modular polyketide synthase structure and function, and highlight recent advances in the characterization and exploitation of these systems.