YALI0E32769g (DGA1) and YALI0E16797g (LRO1) encode major triacylglycerol synthases of the oleaginous yeast Yarrowia lipolytica.
YALI0E32769g (DGA1) and YALI0E16797g (LRO1) encode major triacylglycerol synthases of the oleaginous yeast Yarrowia lipolytica.
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DOI：
10.1016/j.bbalip.2011.07.004
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发表时间：
2011-10
期刊：
影响因子：
--
通讯作者：
Athenstaedt K
中科院分区：
文献类型：
--
作者：
Athenstaedt K
产油酵母解脂耶氏酵母具有类似于高等真核生物脂肪细胞的显著的生产和储存三酰甘油的能力。本文报道了YALI 0 E32769 g（DGA 1）和YALI 0 E16797 g（LRO 1）两个编码解脂耶氏酵母主要三酰甘油脱氢酶的基因的鉴定。在三酰甘油合成缺陷的芽殖酵母酿酒酵母的突变体中，DGA 1或LRO 1的异源表达恢复了这种中性脂质的形成。Dga 1 p需要酰基辅酶A作为二酰基甘油酰化的底物，而Lro 1 p是使用磷脂作为酰基供体的酰基辅酶A非依赖性三酰基甘油合酶。缺失DGAl和/或LRO 1的解脂耶氏酵母菌株在含葡萄糖的培养基上的生长显著降低三酰基甘油积累。最有趣的是，当油酸作为碳源时，突变体与野生型中三酰甘油积累的比率在DGAl缺陷的菌株中显著增加，但在Iro 1 Δ中没有。体外实验表明，在这些条件下，一个额外的酰基辅酶A依赖性三酰甘油合成酶有助于在各自的突变体中的三酰甘油合成。综上所述，提供了解脂耶氏酵母含有至少四种三酰甘油脱氢酶，即Lro 1 p、Dga 1 p和两种另外的三酰甘油脱氢酶的证据，其中一种是酰基辅酶A依赖性的，并且在油酸上生长时特异性诱导。► Y.的2种三酰甘油合酶的鉴定。lipolytica，Dga 1 p和Lro 1 p。Dga 1 p是一种酰基辅酶A依赖性三酰基甘油合成酶。► Lro 1 p使用磷脂作为酰基供体来形成三酰甘油。油酸上的生长诱导额外的三酰甘油合酶。
The oleaginous yeast Yarrowia lipolytica has an outstanding capacity to produce and store triacylglycerols resembling adipocytes of higher eukaryotes. Here, the identification of two genes YALI0E32769g (DGA1) and YALI0E16797g (LRO1) encoding major triacylglycerol synthases of Yarrowia lipolytica is reported. Heterologous expression of either DGA1 or LRO1 in a mutant of the budding yeast Saccharomyces cerevisiae defective in triacylglycerol synthesis restores the formation of this neutral lipid. Whereas Dga1p requires acyl-CoA as a substrate for acylation of diacylglycerol, Lro1p is an acyl-CoA independent triacylglycerol synthase using phospholipids as acyl-donor. Growth of Yarrowia lipolytica strains deleted of DGA1 and/or LRO1 on glucose containing medium significantly decreases triacylglycerol accumulation. Most interestingly, when oleic acid serves as the carbon source the ratio of triacylglycerol accumulation in mutants to wild-type is significantly increased in strains defective in DGA1 but not in lro1Δ. In vitro experiments revealed that under these conditions an additional acyl-CoA dependent triacylglycerol synthase contributes to triacylglycerol synthesis in the respective mutants. Taken together, evidence is provided that Yarrowia lipolytica contains at least four triacylglycerol synthases, namely Lro1p, Dga1p and two additional triacylglycerol synthases whereof one is acyl-CoA dependent and specifically induced upon growth on oleic acid. ►Identification of 2 triacylglycerol synthases of Y. lipolytica, Dga1p and Lro1p. ►Dga1p is an acyl-CoA dependent triacylglycerol synthase. ►Lro1p uses phospholipids as acyl donor for triacylglycerol formation. ►Growth on oleic acid induces additional triacylglycerol synthase(s).
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影响因子：
3.2
作者：
Athenstaedt, K;Zweytick, D;Daum, G
通讯作者：
Daum, G
DOI：
10.1073/pnas.120067297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Dahlqvist, A;St책hl, U;Stymne, H
通讯作者：
Stymne, H
影响因子：
4.8
作者：
Oelkers, P;Tinkelenberg, A;Sturley, SL
通讯作者：
Sturley, SL
影响因子：
4.8
作者：
Connerth, Melanie;Czabany, Tibor;Daum, Guenther
通讯作者：
Daum, Guenther
影响因子：
4.8
作者：
Buhman, KK;Smith, SJ;Farese, RV
通讯作者：
Farese, RV