The expanding role of electrospray ionization mass spectrometry for probing reactive intermediates in solution.
The expanding role of electrospray ionization mass spectrometry for probing reactive intermediates in solution.
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电喷雾电离质谱在探测溶液中的反应中间体方面的扩展作用
DOI：
10.3390/molecules171011507
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发表时间：
2012-09-27
期刊：
影响因子：
--
通讯作者：
Chen B
中科院分区：
文献类型：
--
作者：
Zhu W;Yuan Y;Zhou P;Zeng L;Wang H;Tang L;Guo B;Chen B
在过去的十年中，电喷雾电离质谱（ESI-MS）已迅速占据了液相机理研究的突出地位，由于其固有的优势，允许有效的“钓鱼”（快速，灵敏，特异性和同时检测/识别）的多个中间体和产品直接从“真实世界”的解决方案。在这篇综述中，我们试图提供一个全面的概述的电喷雾质谱为基础的方法和策略，研究反应溶液中的反应物种。本文简要介绍了复杂（生物）化学反应系统中探测反应物种所涉及的一般问题，包括反应中间体（代谢物）产生的潜在来源、分析方面和挑战、ESI-MS的基本原理和最先进的技术。本综述的主要目的是强调ESI-MS的实用性及其在探测溶液中各种反应的活性中间体方面的作用，特别关注基于ESI-MS的方法的当前进展，这些方法通过使用新开发的MS仪器和新兴的电离源（如环境ESI技术）来提高通量，测试真实性和实时检测。此外，还讨论了现代电喷雾质谱在检测有机反应中间体方面的局限性。
Within the past decade, electrospray ionization mass spectrometry (ESI-MS) has rapidly occupied a prominent position for liquid-phase mechanistic studies due to its intrinsic advantages allowing for efficient “fishing” (rapid, sensitive, specific and simultaneous detection/identification) of multiple intermediates and products directly from a “real-world” solution. In this review we attempt to offer a comprehensive overview of the ESI-MS-based methodologies and strategies developed up to date to study reactive species in reaction solutions. A full description of general issues involved with probing reacting species from complex (bio)chemical reaction systems is briefly covered, including the potential sources of reactive intermediate (metabolite) generation, analytical aspects and challenges, basic rudiments of ESI-MS and the state-of-the-art technology. The main purpose of the present review is to highlight the utility of ESI-MS and its expanding role in probing reactive intermediates from various reactions in solution, with special focus on current progress in ESI-MS-based approaches for improving throughput, testing reality and real-time detection by using newly developed MS instruments and emerging ionization sources (such as ambient ESI techniques). In addition, the limitations of modern ESI-MS in detecting intermediates in organic reactions is also discussed.
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