Maintenance of a bone collagen phenotype by osteoblast-like cells in 3D periodic porous titanium (Ti-6Al-4 V) structures fabricated by selective electron beam melting.
Maintenance of a bone collagen phenotype by osteoblast-like cells in 3D periodic porous titanium (Ti-6Al-4 V) structures fabricated by selective electron beam melting.
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DOI：
10.3109/03008207.2013.822864
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发表时间：
2013
影响因子：
2.9
通讯作者：
Fernandes RJ
中科院分区：
文献类型：
--
作者：
Hrabe NW;Heinl P;Bordia RK;Körner C;Fernandes RJ
采用选择性电子束熔炼法（EBM）制备了相对密度（0.17-0.40）和孔径（500-1500 μm）范围内的规则三维周期性多孔Ti-6Al-4V结构。用人成骨细胞样细胞（SAOS-2）接种结构并培养四周。细胞在这些结构内增殖，细胞外基质胶原含量增加。典型地由成骨细胞合成的I型和V型胶原在培养中随时间沉积在新形成的基质中。高放大率扫描电子显微镜显示细胞附着在结构内部的表面上，具有越来越多的纤维基质。体外结果表明，设计用于解决应力屏蔽效应的新型EBM处理的多孔结构有助于成骨细胞附着、增殖和骨胶原基质特征沉积。
Regular 3D periodic porous Ti-6Al-4 V structures were fabricated by the selective electron beam melting method (EBM) over a range of relative densities (0.17–0.40) and pore sizes (500–1500 μm). Structures were seeded with human osteoblast-like cells (SAOS-2) and cultured for four weeks. Cells multiplied within these structures and extracellular matrix collagen content increased. Type I and type V collagens typically synthesized by osteoblasts were deposited in the newly formed matrix with time in culture. High magnification scanning electron microscopy revealed cells attached to surfaces on the interior of the structures with an increasingly fibrous matrix. The in-vitro results demonstrate that the novel EBM-processed porous structures, designed to address the effect of stress-shielding, are conducive to osteoblast attachment, proliferation and deposition of a collagenous matrix characteristic of bone.