ULTRASONIC ENHANCEMENT OF ANTIBIOTIC ACTION ON GRAM-NEGATIVE BACTERIA
ULTRASONIC ENHANCEMENT OF ANTIBIOTIC ACTION ON GRAM-NEGATIVE BACTERIA
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DOI：
10.1128/aac.38.11.2577
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发表时间：
1994-11-01
影响因子：
4.9
通讯作者：
SAGERS, RD
中科院分区：
文献类型：
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作者：
PITT, WG;MCBRIDE, MO;SAGERS, RD
在施加和不施加67 kHz超声刺激的情况下测量了庆大霉素对铜绿假单胞菌、大肠杆菌、表皮葡萄球菌和金黄色葡萄球菌浮游培养物的影响。超声波施加在对细菌没有抑制或杀菌活性的水平。测定庆大霉素对铜绿假单胞菌和大肠杆菌的最低抑菌浓度和杀菌活性。大肠杆菌的研究表明，同时应用67 kHz的超声波增强了抗生素的有效性。当庆大霉素浓度和超声水平结合时，观察到协同效应，细菌活力降低了几个数量级，而超声水平本身并不降低活力。随着培养时间的增加，细菌对单独抗生素的作用更具抵抗力。超声波的应用似乎扭转了这种阻力。超声波处理增强的活性在铜绿假单胞菌和大肠杆菌的培养物中是明显的。大肠埃希菌培养物中未观察到革兰氏阳性S. epidermidis和S.金黄色。这些结果可应用于治疗植入器械上的细菌生物膜感染，这些感染通常对抗生素治疗更具抗性。
The effect of gentamicin upon planktonic cultures of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus epidermidis, and Staphylococcus aureus was measured with and without application of 67-kHz ultrasonic stimulation. The ultrasound was applied at levels that had no inhibitory or bactericidal activity against the bacteria. Measurements of the MIC and bactericidal activity of gentamicin against planktonic cultures of P. aeruginosa and E. coli demonstrated that simultaneous application of 67-kHz ultrasound enhanced the effectiveness of the antibiotic. A synergistic effect was observed and bacterial viability was reduced several orders of magnitude when gentamicin concentrations and ultrasonic levels which by themselves did not reduce viability were combined. As the age of the culture increased, the bacteria became more resistant to the effect of the antibiotic alone. Application of ultrasound appeared to reverse this resistance. The ultrasonic treatment-enhanced activity was evident with cultures of P. aeruginosa and E. coli but was not observed with cultures of gram-positive S. epidermidis and S. aureus. These results may have application in the treatment of bacterial biofilm infections on implant devices, which infections are usually more resistant to antibiotic therapy.